Clinical relevance of tertiary lymphoid structures in esophageal squamous cell carcinoma

Sota Deguchi; Hiroaki Tanaka; Shugo Suzuki; Seji Natsuki; Takuya Mori; Yuichiro Miki; Mami Yoshii; Tatsuro Tamura; Takahiro Toyokawa; Shigeru Lee; Kazuya Muguruma; Hideki Wanibuchi; Masaichi Ohira

doi:10.1186/s12885-022-09777-w

Clinical relevance of tertiary lymphoid structures in esophageal squamous cell carcinoma

BMC Cancer. 2022 Jun 24;22(1):699. doi: 10.1186/s12885-022-09777-w.

Affiliations

¹ Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, 1-4-3, Asahimachi, Abenoku, Osaka, 545-8585, Japan.
² Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, 1-4-3, Asahimachi, Abenoku, Osaka, 545-8585, Japan. hiroakitan@omu.ac.jp.
³ Department of Molecular Pathology, Osaka City University Graduate School of Medicine, Osaka, Japan.

Abstract

Background: Tertiary lymphoid structures (TLSs) have been reported to be involved in immune responses in many carcinomas. This study investigated the significance of TLSs in esophageal squamous cell carcinoma, focusing on TLS maturation. METHODS: The relationships of TLSs with clinicopathological features of 236 patients who underwent curative surgery for stage 0-IV esophageal squamous cell carcinoma were investigated. Mature TLSs, in which the germinal center formation was rich in CD23⁺ cells, were classified as TLSs containing a germinal center (GC-TLSs). GC-TLS densities were measured, and CD8⁺ cells were counted. The prognostic impact of GC-TLSs was assessed by Kaplan-Meier plots using the log-rank test for the relapse-free survival. A comparative study of GC-TLSs was performed using the Wilcoxon rank sum test. The relationship between GC-TLSs and CD8⁺ cells was examined by Spearman's rank correlation coefficient test.

Results: TLSs were located mainly at the invasive margin of the tumor in cases with esophageal squamous cell carcinoma. Among the patients treated with neoadjuvant chemotherapy, those with advanced disease had a better prognosis in the GC-TLS high-density group than did those in the GC-TLS low-density group. Patients in whom neoadjuvant chemotherapy was effective had more GC-TLSs than those in whom it was less effective. The density of GC-TLSs and the number of tumor-infiltrating CD8⁺ cells were higher in patients treated with neoadjuvant chemotherapy than in those without chemotherapy, and a weak correlation between the density of GC-TLSs and the number of tumor-infiltrating CD8⁺ cells was observed. Moreover, co-culturing of PBMCs with an anticancer drug-treated esophageal squamous cell carcinoma cell line increased the CD20 and CD23 expression in PBMCs in vitro.

Conclusion: TLS maturation may be important for evaluating the local tumor immune response in patients treated with neoadjuvant chemotherapy for esophageal squamous cell carcinoma. The present results suggest that TLS maturation may be a useful target for predicting the efficacy of immunotherapy, including immune checkpoint inhibitor treatment for esophageal squamous cell carcinoma.

Keywords: B cell; Chemotherapy; Esophageal squamous cell carcinoma; Immunotherapy; Tertiary lymphoid structures.

MeSH terms

Esophageal Neoplasms* / pathology
Esophageal Squamous Cell Carcinoma* / pathology
Humans
Lymphocytes, Tumor-Infiltrating
Neoplasm Recurrence, Local / pathology
Prognosis
Tertiary Lymphoid Structures* / metabolism
Tertiary Lymphoid Structures* / pathology
Tumor Microenvironment