Determination of Volatile Compounds of Mentha piperita and Lavandula multifida and Investigation of Their Antibacterial, Antioxidant, and Antidiabetic Properties

Samiah Hamad Al-Mijalli; Eman R ELsharkawy; Emad M Abdallah; Munerah Hamed; Nasreddine El Omari; Shafi Mahmud; Mohammed Merae Alshahrani; Hanae Naceiri Mrabti; Abdelhakim Bouyahya

doi:10.1155/2022/9306251

Determination of Volatile Compounds of Mentha piperita and Lavandula multifida and Investigation of Their Antibacterial, Antioxidant, and Antidiabetic Properties

Evid Based Complement Alternat Med. 2022 Jun 14:2022:9306251. doi: 10.1155/2022/9306251. eCollection 2022.

Authors

Samiah Hamad Al-Mijalli¹, Eman R ELsharkawy², Emad M Abdallah³, Munerah Hamed⁴, Nasreddine El Omari⁵, Shafi Mahmud⁶, Mohammed Merae Alshahrani⁷, Hanae Naceiri Mrabti⁸, Abdelhakim Bouyahya⁹

Affiliations

¹ Department of Biology, College of Sciences, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
² Chemistry Department, Northern Border University Saudi Arabia, Arar, Saudi Arabia.
³ Department of Science Laboratories, College of Science and Arts, Qassim University, Ar Rass, Saudi Arabia.
⁴ Department of Pathology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia.
⁵ Laboratory of Histology, Embryology, and Cytogenetic, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat, Morocco.
⁶ Division of Genome Sciences and Cancer, The John Curtin School of Medical Research and The Shine-Dalgarno Centre for RNA Innovation, The Australian National University, Canberra, ACT 2601, Australia.
⁷ Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, 1988, Najran 61441, Saudi Arabia.
⁸ Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analysis Research Team, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, BP 6203, Rabat, Morocco.
⁹ Laboratory of Human Pathologies Biology, Department of Biology, Faculty of Sciences, and Genomic Center of Human Pathologies, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat, Morocco.

Abstract

Mentha piperita and Lavandula multifida are widely used in Moroccan traditional medicine for the treatment of diabetes and infectious diseases. The aims of this work were the determination of the chemical composition of Mentha piperita (MPEO) and Lavandula multifida (LMEO) essential oils and the evaluation of their antibacterial, antioxidant, and antidiabetic activities. The chemical composition was determined by GC-MS analysis. The antibacterial effects were evaluated against several bacterial strains using disc diffusion, MIC, and MBC methods. The antioxidant activity was evaluated in vitro using DPPH, H₂O₂, and xanthine oxidase, and the antidiabetic activity was estimated by the inhibitory effects of α-amylase, α-glucosidase, and lipase activities. GC-MS results showed that the main compounds of MPEO were menthone (29.24%), levomenthol (38.73%), and eucalyptol (6.75%). However, eucalyptol (28.11%), 2-bornanone (11.57%), endo-borneol (7.82%), and linalyl acetate (5.22%) are the major compounds of LMEO. The results exhibited important inhibitory effects against some bacterial strains with MIC = MBC = 0.39 mg/mL for MPEO against Staphylococcus aureus ATCC. However, LMEO exhibited remarkable antioxidant and antidiabetic activities compared to MPEO. Indeed, LMEO inhibited DPPH, H₂O₂, and xanthine oxidase with concentrations of 15.23, 21.52, and 8.89 µg/mL, respectively. Moreover, LMEO exhibited α-amylase and α-glucosidase at IC₅₀ = 85.34 and IC₅₀ = 59.36 µg/mL, respectively. The findings showed that both MPEO and LMEO exhibit promising biological properties. However, the application of these species or their main bioactive compounds requires further investigation.