Alpha-tocopherol-loaded polycaprolactone nanoparticles improve the inflammation and systemic oxidative stress of arthritic rats

Lucas S Moreira; Any Carolina Chagas; Ana Paula Ames-Sibin; Vanesa O Pateis; Odinei H Gonçalves; Francielli Maria S Silva-Comar; Luzmarina Hernandes; Anacharis B Sá-Nakanishi; Lívia Bracht; Ciomar A Bersani-Amado; Adelar Bracht; Jurandir F Comar

doi:10.1016/j.jtcme.2021.12.003

Alpha-tocopherol-loaded polycaprolactone nanoparticles improve the inflammation and systemic oxidative stress of arthritic rats

J Tradit Complement Med. 2021 Dec 24;12(4):414-425. doi: 10.1016/j.jtcme.2021.12.003. eCollection 2022 Jul.

Affiliations

¹ Department of Biochemistry, State University of Maringá, PR, Brazil.
² Post-Graduation Program of Food Technology - Federal University of Technology - Paraná, Campo Mourão, PR, Brazil.
³ Department of Pharmacology and Therapeutics, State University of Maringá, PR, Brazil.
⁴ Department of Morphological Sciences, State University of Maringá, PR, Brazil.

Abstract

Background and aim: The present study investigated the effects of orally administered α-tocopherol-loaded polycaprolactone nanoparticles on the articular inflammation and systemic oxidative status of middle-aged Holtzman rats with Freund's adjuvant-induced polyarthritis, a model for rheumatoid arthritis. Intraperitoneally administered free α-tocopherol provided the reference for comparison.

Experimental procedure: Two protocols of treatment were followed: intraperitoneal administration of free α-tocopherol (100 mg/kg i.p.) or oral administration of free and nanoencapsulated α-tocopherol (100 mg/kg p.o.). Animals were treated during 18 days after arthritis induction.

Results: Free (i.p.) and encapsulated α-tocopherol decreased the hind paws edema, the leukocytes infiltration into femorotibial joints and the mRNA expression of pro-inflammatory cytokines in the tibial anterior muscle of arthritic rats, but the encapsulated compound was more effective. Free (i.p.) and encapsulated α-tocopherol decreased the high levels of reactive oxygen species in the brain and liver, but only the encapsulated compound decreased the levels of protein carbonyl groups in these organs. Both free (i.p.) and encapsulated α-tocopherol increased the α-tocopherol levels and the ratio of reduced to oxidized glutathione in these organs.

Conclusion: Both intraperitoneally administered free α-tocopherol and orally administered encapsulated α-tocopherol effectively improved inflammation and systemic oxidative stress in middle-aged arthritic rats. However, the encapsulated form should be preferred because the oral administration route does not be linked to the evident discomfort that is caused in general by injectable medicaments. Consequently, α-tocopherol-loaded polycaprolactone nanoparticles may be a promising adjuvant to the most current approaches aiming at rheumatoid arthritis therapy.

Keywords: Adjuvant-induced arthritis; Antioxidant status; Oxidative status; Polycaprolactone; Rheumatoid arthritis; α-tocopherol.