Metabolomics Analysis of PK-15 Cells with Pseudorabies Virus Infection Based on UHPLC-QE-MS

Panrao Liu; Danhe Hu; Lili Yuan; Zhengmin Lian; Xiaohui Yao; Zhenbang Zhu; Xiangdong Li

doi:10.3390/v14061158

Metabolomics Analysis of PK-15 Cells with Pseudorabies Virus Infection Based on UHPLC-QE-MS

Viruses. 2022 May 27;14(6):1158. doi: 10.3390/v14061158.

Authors

Panrao Liu¹, Danhe Hu¹, Lili Yuan¹, Zhengmin Lian¹, Xiaohui Yao¹, Zhenbang Zhu¹, Xiangdong Li^{1

2}

Affiliations

¹ Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
² Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.

Abstract

Viruses depend on the metabolic mechanisms of the host to support viral replication. We utilize an approach based on ultra-high-performance liquid chromatography/Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass (UHPLC-QE-MS) to analyze the metabolic changes in PK-15 cells induced by the infections of the pseudorabies virus (PRV) variant strain and Bartha K61 strain. Infections with PRV markedly changed lots of metabolites, when compared to the uninfected cell group. Additionally, most of the differentially expressed metabolites belonged to glycerophospholipid metabolism, sphingolipid metabolism, purine metabolism, and pyrimidine metabolism. Lipid metabolites account for the highest proportion (around 35%). The results suggest that those alterations may be in favor of virion formation and genome amplification to promote PRV replication. Different PRV strains showed similar results. An understanding of PRV-induced metabolic reprogramming will provide valuable information for further studies on PRV pathogenesis and the development of antiviral therapy strategies.

Keywords: PK-15 cells; UHPLC-QE-MS; metabolomic analysis; pseudorabies virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatography, High Pressure Liquid
Herpesvirus 1, Suid* / genetics
Metabolomics
Pseudorabies*
Swine
Swine Diseases*

Grants and funding

This work was funded by the National Natural Science Foundation of China (Nos. 32102637 and 32172823) and the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).