Brooding Temperature Alters Yolk Sac Absorption and Affected Ovarian Development in Goslings

Zhengquan Liu; Xingyong Chen; Yutong Zhao; Jingzhou Peng; Daoyou Chen; Shiqi Yu; Zhaoyu Geng

doi:10.3390/ani12121513

Brooding Temperature Alters Yolk Sac Absorption and Affected Ovarian Development in Goslings

Animals (Basel). 2022 Jun 10;12(12):1513. doi: 10.3390/ani12121513.

Authors

Zhengquan Liu¹, Xingyong Chen^{1

2}, Yutong Zhao¹, Jingzhou Peng¹, Daoyou Chen¹, Shiqi Yu¹, Zhaoyu Geng^{1

2}

Affiliations

¹ College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, China.
² Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, China.

Abstract

In order to explore the brooding temperature on the absorption of yolk sac and the ovary development of goslings, 126 1-day-old female goslings were randomly divided into three groups with three replicates in each group. The brooding temperatures were set at 32 °C, 29 °C and 26 °C (represent G32, G29 and G26), respectively, in each group. At 48, 60 and 72 h, two goslings from each replicate were weighed, and the yolk sac was collected and weighed. The fatty acid composition of yolk sac fluid was determined by gas chromatography-mass spectrometry (GC-MS). At 1, 2, 3, and 4 weeks of age, goslings from each replicate were weighed, the ovaries were weighed and fixed for hematoxylin-eosin (HE) staining, Cell cycle checkpoint kinase 1 (CHK1), fibroblast growth factor 12 (FGF12) and Sma-and Mad-related protein 4 (SMAD4) which related to regulation of ovarian development were determined by qRT-PCR. The body weight of G29 and G26 was significantly higher than that of G32 at 72 h (p < 0.05). The contents of C14:0, C16:0, C18:2n6c and total fatty acid (ΣTFA) from G32 were significantly higher than that of G26 (p < 0.05), and the contents of C18:1n9t and C22:0 in G29 were significantly higher than that of G26 (p < 0.05). The ovary index, ovary and body weight were significantly higher in G29 than those of G32 and G26 at 2 weeks of age (p < 0.05). The number of primordial follicles, number of primary follicles and diameter of primary follicles were significantly higher in G29 than those in G32 and G26 at 4 weeks of age (p < 0.05). In G29, the expression of CHK1 and SMAD4 was significantly higher than that in G32, and the expression of FGF12 and SMAD4 was significantly higher (p < 0.05) than that in G26 at 2 and 4 weeks of age. In conclusion, brooding temperature at 29 °C could promote the absorption of fatty acids in yolk sac, body weight gain, and ovarian development through up-regulating the expression of CHK1, FGF12 and SMAD4.

Keywords: brooding temperature; gosling; ovary; yolk sac.

Grants and funding

This research was financially funded by the Key Project of National Science and Technology of Xinjiang Autonomous Region (2020B01004-2), Graduate scientific research project of Anhui Provincial Education Institute (YJS20210224), and Scientific research project of Anhui Provincial Education Institute (KJ2020A0891).