Liza ramada Juveniles after Exposure to the Toxic Dinoflagellate Vulcanodinium rugosum: Effects on Fish Viability, Tissue Contamination and Microalgae Survival after Gut Passage

Aurélien Bouquet; Marie Anaïs Perdrau; Mohamed Laabir; Elodie Foucault; Nicolas Chomérat; Jean Luc Rolland; Eric Abadie

doi:10.3390/toxins14060401

Liza ramada Juveniles after Exposure to the Toxic Dinoflagellate Vulcanodinium rugosum: Effects on Fish Viability, Tissue Contamination and Microalgae Survival after Gut Passage

Toxins (Basel). 2022 Jun 10;14(6):401. doi: 10.3390/toxins14060401.

Authors

Aurélien Bouquet¹, Marie Anaïs Perdrau¹, Mohamed Laabir², Elodie Foucault¹, Nicolas Chomérat³, Jean Luc Rolland¹, Eric Abadie^{1

4}

Affiliations

¹ MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, 87 Avenue Jean Monnet, 34200 Sète, France.
² MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, 34095 Montpellier, France.
³ IFREMER, Station de Biologie Marine, Place de la Croix, 29900 Concarneau, France.
⁴ IFREMER, Biodivenv, 79 Route de Pointe Fort, 97231 Martinique, France.

Abstract

Pinnatoxins (PnTX) and Portimines (Prtn), two toxins produced by the benthic dinoflagellate Vulcanodinium rugosum, are known to be lethal to mice after intraperitoneal or oral administration. They are also known to accumulate in shellfish such as mussels and clams, but their effect on fish and the upper food chain remains unknown. In this work, juveniles of the fish Liza ramada (Mullet) were exposed to a strain of V. rugosum producing PnTX G and Prtn A. The fishes' viability and contamination were recorded at times interval. Results showed that L. ramada juveniles were able to feed on V. rugosum and that their tissues could be contaminated by PnTX G and Prtn A without impact on fish viability. Furthermore, the microalgae temporary cysts survived and germinated after fish gut passage. This study showed the potential of L. ramada to transfer PnTX and Prtn toxins to the upper food chain and to disseminate V. rugosum in environment.

Keywords: Liza ramada; Vulcanodinium rugosum; contamination; dissemination; fish; food chain; pinnatoxins; portimines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bivalvia*
Dinoflagellida*
Fishes
Mice
Microalgae*
Smegmamorpha*
Tissue Survival

Grants and funding

This research was supported by MARBEC (MARine Biodiversity, Exploitation and Conservation) and by the French Ministry of Agriculture and Food. This research received no external funding.