Ecophysiological Responses of Tall Wheatgrass Germplasm to Drought and Salinity

Celina I Borrajo; Adela M Sánchez-Moreiras; Manuel J Reigosa

doi:10.3390/plants11121548

Ecophysiological Responses of Tall Wheatgrass Germplasm to Drought and Salinity

Plants (Basel). 2022 Jun 10;11(12):1548. doi: 10.3390/plants11121548.

Authors

Celina I Borrajo^{1

2}, Adela M Sánchez-Moreiras¹, Manuel J Reigosa¹

Affiliations

¹ Departamento de Bioloxía Vexetal e Ciencias do Solo, Facultade de Bioloxía, Universidade de Vigo, Campus Lagoas Marcosende s/n, 36310 Vigo, Spain.
² Agricultural Experimental Station Cuenca del Salado of INTA (National Institute of Agricultural Technology), Av. Belgrano 416, Rauch 7203, Argentina.

Abstract

Tall wheatgrass (Thinopyrum ponticum (Podp.) Barkworth and D.R. Dewey) is an important, highly salt-tolerant C3 forage grass. The objective of this work was to learn about the ecophysiological responses of accessions from different environmental origins under drought and salinity conditions, to provide information for selecting superior germplasm under combined stress in tall wheatgrass. Four accessions (P3, P4, P5, P9) were irrigated using combinations of three salinity levels (0, 0.1, 0.3 M NaCl) and three drought levels (100%, 50%, 30% water capacity) over 90 days in a greenhouse. The control treatment showed the highest total biomass, but water-use efficiency (WUE), δ¹³C, proline, N concentration, leaf length, and tiller density were higher under moderate drought or/and salinity stress than under control conditions. In tall wheatgrass, K⁺ functions as an osmoregulator under drought, attenuated by salinity, and Na⁺ and Cl^- function as osmoregulators under salinity and drought, while proline is an osmoprotector under both stresses. P3 and P9, from environments with mild/moderate stress, prioritized reproductive development, with high evapotranspiration and the lowest WUE and δ¹³C values. P4 and P5, from more stressful environments, prioritized vegetative development through tillering, showing the lowest evapotranspiration, the highest δ¹³C values, and different mechanisms for limiting transpiration. The δ¹³C value, leaf biomass, tiller density, and leaf length had high broad-sense heritability (H²), while the Na⁺/K⁺ ratio had medium H². In conclusion, the combined use of the δ¹³C value, Na⁺/K⁺ ratio, and canopy structural variables can help identify accessions that are well-adapted to drought and salinity, also considering the desirable plant characteristics. Tall wheatgrass stress tolerance could be used to expand forage production under a changing climate.

Keywords: 13-carbon isotope; C3 grass; K+ concentration; Na+ and Cl− concentration; Na+/K+ ratio; combined stress; leaf length; proline; tiller density; water-use efficiency.

Grants and funding

This work was financially supported by the Xunta de Galicia, grant number ED431C 2019/20, for the project ‘Consolidación e estruturación de unidades de investigación competitivas do Sistema Universitario de Galicia. Grupos de referencia competitiva’.