Development and Validation of Antibodies Targeting Site-Specific Histone Methylation

Lara Zorro Shahidian; Sylvain Daujat

doi:10.1007/978-1-0716-2481-4_9

Development and Validation of Antibodies Targeting Site-Specific Histone Methylation

Methods Mol Biol. 2022:2529:171-206. doi: 10.1007/978-1-0716-2481-4_9.

Authors

Lara Zorro Shahidian¹, Sylvain Daujat²

Affiliations

¹ Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), University of Cantabria, Santander, Spain.
² Biotechnology and Cell Signaling, CNRS UMR7242, University of Strasbourg, Illkirch Cedex, France. sdaujat@unistra.fr.

PMID: 35733016
DOI: 10.1007/978-1-0716-2481-4_9

Abstract

The development of specific anti-modification antibodies as research tools has revolutionized the way histone methylation is studied. Lack of stringent quality controls, however, led to the development of nonspecific antibodies, compromising their use. In this chapter, we provide a series of protocols that collectively will help those studying histone methylation to develop and thoroughly validate high-end sequence-specific and methylation-dependent antibodies.

Keywords: Affinity purification; Antibody validation; Histone methylation; Rabbit polyclonal antibodies; Site-specific antibody development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / metabolism
Histones* / metabolism
Methylation
Protein Processing, Post-Translational*

Substances

Antibodies
Histones