From Methylome to Integrative Analysis of Tissue Specificity

Methods Mol Biol. 2022:2505:223-240. doi: 10.1007/978-1-0716-2349-7_16.

Abstract

DNA methylation is the most studied epigenetic mark in both plants and animals. The gold standard for assaying genome-wide DNA methylation at single-base resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant Catharanthus roseus.

Keywords: Catharanthus roseus; DNA methylation; Methylome pipeline; Ovation Methyl-seq; Whole-Genome Bisulfite Sequencing (WGBS).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA / genetics
  • DNA Methylation
  • Epigenome*
  • Epigenomics / methods
  • High-Throughput Nucleotide Sequencing* / methods
  • Organ Specificity / genetics
  • Sequence Analysis, DNA / methods
  • Sulfites

Substances

  • Sulfites
  • DNA