TARGETing Transcriptional Regulation in the Medicinal Plant Catharanthus roseus

Methods Mol Biol. 2022:2505:191-202. doi: 10.1007/978-1-0716-2349-7_14.

Abstract

Transcriptional regulation is a central piece of the highly valuable monoterpenoid indole alkaloid pathway of C. roseus , and the ultimate tool for its understanding and manipulation. Here, we describe the adaptation of the TARGET methodology to identify specific and genome-wide leaf targets of C. roseus candidate transcription factors (TFs).

Keywords: Catharanthus roseus; FACS; Monoterpenoid indole alkaloids (MIAs); Protoplasts; TARGET; Transcription factors (TFs).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catharanthus* / genetics
  • Catharanthus* / metabolism
  • Gene Expression Regulation, Plant
  • Indole Alkaloids / metabolism
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Medicinal* / genetics
  • Plants, Medicinal* / metabolism

Substances

  • Indole Alkaloids
  • Plant Proteins