Developments and prospects for doubled haploid wheat

Serik Eliby; Sara Bekkuzhina; Olena Kishchenko; Gulnur Iskakova; Gulnar Kylyshbayeva; Satyvaldy Jatayev; Kathleen Soole; Peter Langridge; Nikolai Borisjuk; Yuri Shavrukov

doi:10.1016/j.biotechadv.2022.108007

Developments and prospects for doubled haploid wheat

Biotechnol Adv. 2022 Nov:60:108007. doi: 10.1016/j.biotechadv.2022.108007. Epub 2022 Jun 19.

Authors

Affiliations

¹ University of Adelaide, Urrbrae, SA, Australia.
² Faculty of Agronomy, S.Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan.
³ Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Huai'an, China; Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Kyiv, Ukraine.
⁴ Kazakh Agrarian National University, Almaty, Kazakhstan; Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan.
⁵ Central Asian Innovation University, Shymkent, Kazakhstan.
⁶ College of Science and Engineering, Biological Sciences, Flinders University, SA, Australia.
⁷ University of Adelaide, Urrbrae, SA, Australia; Wheat Initiative, Julius-Kühn-Institute, Berlin, Germany.
⁸ Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Huai'an, China.
⁹ College of Science and Engineering, Biological Sciences, Flinders University, SA, Australia. Electronic address: yuri.shavrukov@flinders.edu.au.

PMID: 35732257
DOI: 10.1016/j.biotechadv.2022.108007

Abstract

Doubled haploid production is a valuable biotechnology that can accelerate the breeding of new wheat varieties by several years through the one-step creation of 100% homozygous plants. The technology also plays important role in studying the genetic control of traits in wheat, in marker-assisted selection, in genomics and in genetic engineering. In this paper, recent advances in androgenesis and gynogenesis techniques, emphasizing predominantly the in vitro culture phase, as well as the emerging innovative approaches in researching and producing wheat doubled haploids are reviewed. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based genome editing, that allows targeted mutagenesis and gene targeting, is being tested extensively as a powerful and precise tool to induce doubled haploids in wheat. The review provides the reader with recent examples of gene modifications in wheat to induce haploidy.

Keywords: Androgenesis; CRISPR; Doubled haploids; Gynogenesis; Haploid inducers.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Clustered Regularly Interspaced Short Palindromic Repeats
Gene Editing / methods
Haploidy
Plant Breeding* / methods
Triticum* / genetics