In Vitro Assays for the Bifunctional Acylpeptide Hydrolase (APEH) Enzyme from Antarctic Fish

Methods Mol Biol. 2022:2498:413-424. doi: 10.1007/978-1-0716-2313-8_25.

Abstract

The bifunctional enzyme acylpeptide hydrolase (APEH) is involved in important metabolic processes both as an exopeptidase and as an endopeptidase. Hence, the growing interest in the study of this protein and the need to set up in vitro assays for its characterization. This chapter describes two in vitro assays able to detect the activities of APEH, one for the exopeptidase activity and one for the endopeptidase activity. In particular, these assays have been set up on the two APEH isoforms from Antarctic fish, characterized by a distinct functionality and marked exo- and endopeptidase activities.

Keywords: Acylpeptide hydrolase (APEH); Antarctic fish; In vitro assay; Oxidized protein hydrolase (OPH).

MeSH terms

  • Animals
  • Antarctic Regions
  • Endopeptidases / metabolism
  • Exopeptidases / metabolism
  • Fishes* / metabolism
  • Peptide Hydrolases* / metabolism
  • Proteolysis

Substances

  • Endopeptidases
  • Exopeptidases
  • Peptide Hydrolases
  • acylaminoacyl-peptidase