Fractionation Protocol of Marine Metabolites

Genoveffa Nuzzo; Emiliano Manzo; Carmela Gallo; Giuliana d'Ippolito; Angelo Fontana

doi:10.1007/978-1-0716-2313-8_16

Fractionation Protocol of Marine Metabolites

Methods Mol Biol. 2022:2498:307-313. doi: 10.1007/978-1-0716-2313-8_16.

Authors

Genoveffa Nuzzo¹, Emiliano Manzo², Carmela Gallo², Giuliana d'Ippolito², Angelo Fontana²

Affiliations

¹ National Research Council of Italy, Institute of Biomolecular Chemistry, Pozzuoli, Naples, Italy. nuzzo.genoveffa@icb.cnr.it.
² National Research Council of Italy, Institute of Biomolecular Chemistry, Pozzuoli, Naples, Italy.

PMID: 35727552
DOI: 10.1007/978-1-0716-2313-8_16

Abstract

Marine organisms are well known for their capability to produce chemically unique and biological relevant small molecules. However, marine extracts are complex mixtures of compounds with a huge presence of salts. Thus, biological screening and chemical analysis of marine extracts pose specific technical constraints and require adequate sample preparation. We have developed an automated solid-phase extraction (SPE)-based method to desalt marine extracts and recover metabolites, adapt to be integrated in platform of high-throughput screening. The procedure uses a poly(styrene-divynylbenzene)-based support and a stepwise organic solvent elution herein described.

Keywords: Chromatographic fractionation; Marine natural product; Secondary metabolites; Solid-phase extraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aquatic Organisms
Chemical Fractionation* / methods
Solid Phase Extraction* / methods
Solvents

Substances

Solvents