Barcoding of Antarctic Marine Invertebrates: From Field Sampling to Lab Procedures

Methods Mol Biol. 2022:2498:177-194. doi: 10.1007/978-1-0716-2313-8_9.

Abstract

DNA barcoding is a powerful and widespread method used to identify large numbers of species collected in the framework of sampling activities in the field. With the exception of research projects that may count on large teams characterized by tasks' delegation and where many activities may run in parallel, in the majority of cases the barcoding effort is handled by a limited number of persons. The guidelines here reported focus on this second case, with a special attention paid to field procedures, whose efficiency and smoothness are often overlooked.

Keywords: Antarctica; Coastal sampling; DNA barcoding; Monitoring.

MeSH terms

  • Animals
  • Antarctic Regions
  • Aquatic Organisms* / genetics
  • DNA / genetics
  • DNA Barcoding, Taxonomic* / methods
  • Invertebrates / genetics

Substances

  • DNA