18F-FDG and 68 Ga-FAPI PET/CT for the evaluation of periprosthetic joint infection and aseptic loosening in rabbit models

Abstract

Purpose: We built a joint replacement loosening model based on the original rabbit model of infection and evaluated the performance characteristics of ¹⁸F-FDG and ⁶⁸ Ga-FAPI in evaluating infection and loosening.

Methods: After surgery, the rabbits were divided into four groups, with six individuals in the control group and 10 each in the aseptic loosening, S. aureus and S. epidermidis groups. PET/CT and serological examination were performed three times at two-week intervals. After the rabbits were euthanized, micro-CT, tissue pathology, pullout tests and scanning electron microscopy (SEM) were performed.

Results: The pullout test and SEM showed the feasibility of the aseptic loosening model. ¹⁸F-FDG showed similar performance in the control and loosening groups. The SUVmax of the S. aureus group was consistently higher than that of the S. epidermidis group. As for ⁶⁸ Ga-FAPI, the SUVmax of the control group was lowest in the second week and gradually increased over subsequent weeks. The SUVmax of the loosening group began to exceed that of the control group after the second week. The SUVmax of the S. aureus group in the second week was the lowest among the four groups and increased as the number of weeks increased. The pathology results showed concordance with the performance of PET/CT. Linear regressions between SUVmax and serology showed that ¹⁸F-FDG was positively correlated with CRP and IL-6, while ⁶⁸ Ga-FAPI revealed negative correlations with CRP and IL-6 in the second week and positive correlations in the sixth week. In addition, the SUVmax and MT(target)V of both ¹⁸F-FDG and ⁶⁸ Ga-FAPI were negatively correlated with bone volume/trabecular volume (TV) and bone surface area/TV.

Conclusion: In this longitudinal observation, ⁶⁸ Ga-FAPI showed greater sensitivity than ¹⁸F-FDG in detecting diseases, and ⁶⁸ Ga-FAPI had no intestinal or muscular uptake. The MT(target)V of ⁶⁸ Ga-FAPI was larger than that of ¹⁸F-FDG, which meant that ⁶⁸ Ga-FAPI had the potential to define the scope of lesions more accurately. Finally, the SUVmax of ⁶⁸ Ga-FAPI could not differentiate between loosening and infection; further study of the diagnostic criteria is warranted.

Keywords: 18F-FDG; 68 Ga-FAPI; Aseptic loosening; Periprosthetic joint infection; Rabbit model.