Preparation of a biomimetic bi-layer chitosan wound dressing composed of A-PRF/sponge layer and L-arginine/nanofiber

Marjan Mirhaj; Mohamadreza Tavakoli; Jaleh Varshosaz; Sheyda Labbaf; Saeideh Salehi; Ardeshir Talebi; Nafise Kazemi; Vida Haghighi; Mansoor Alizadeh

doi:10.1016/j.carbpol.2022.119648

Preparation of a biomimetic bi-layer chitosan wound dressing composed of A-PRF/sponge layer and L-arginine/nanofiber

Carbohydr Polym. 2022 Sep 15:292:119648. doi: 10.1016/j.carbpol.2022.119648. Epub 2022 May 23.

Authors

Marjan Mirhaj¹, Mohamadreza Tavakoli¹, Jaleh Varshosaz², Sheyda Labbaf³, Saeideh Salehi⁴, Ardeshir Talebi⁵, Nafise Kazemi⁴, Vida Haghighi⁶, Mansoor Alizadeh⁷

Affiliations

¹ Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
² Department of Pharmaceutics, Novel Drug Delivery Systems Research Centre, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran. Electronic address: varshosaz@pharm.mui.ac.ir.
³ Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: s.labbaf@iut.ac.ir.
⁴ Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
⁵ Department of Pathology, Faculty of Medicine, Isfahan University of Medical Science, Isfahan, Iran. Electronic address: talebi@med.mui.ac.ir.
⁶ Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
⁷ Department of Biomedical Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran.

PMID: 35725158
DOI: 10.1016/j.carbpol.2022.119648

Abstract

To better mimic the structure of skin tissue, the use of a multi-layered wound dressing has been proposed. In the present study, a sponge-nanofibrous bi-layer dressing is designed. For this purpose, a chitosan/polyethylene glycol (CsPEG) sponge with advanced platelet-rich fibrin (A-PRF) was prepared as the upper layer of wound dressing, and a Cs/L-arginine electrospun nanofiber layer as the bottom layer. After physical, chemical and mechanical evaluations, the release of platelet-derived growth factor-AB (PDGF-AB), vascular endothelial growth factor (VEGF) and L-arginine were investigated. The antibacterial activity, cell viability and attachment of Bi-layer1.5 dressing (CsPEG/1.5A-PRF sponge coated with Cs/0.5 L-arginine nanofibers) were significantly higher than other dressings. Also, Bi-layer1.5 dressing increased the angiogenic potential and accelerated the wound healing, compared to other samples. Given the promising obtained results, the use of Bi-layer1.5 wound dressing with the ability to release growth factors and L-arginine is highly recommended to treat full-thickness wounds.

Keywords: Advanced platelet-rich fibrin; Bi-layer; Chitosan; L-arginine; Nanofiber; Sponge; Wound dressing.

MeSH terms

Anti-Bacterial Agents / pharmacology
Arginine
Bandages
Biomimetics
Chitosan* / chemistry
Nanofibers* / chemistry
Platelet-Rich Fibrin*
Vascular Endothelial Growth Factor A

Substances

Anti-Bacterial Agents
Vascular Endothelial Growth Factor A
Chitosan
Arginine