Compositional Analysis and Potent Insecticidal Activity of Supercritical CO2 Fluid Extracts of Alcea nudiflora L. Leaves

Nazira K Khidyrova; Saida M Turaeva; Malohat J Rakhmatova; Khayrulla M Bobakulov; Shamansur S Sagdullaev; Rano P Zakirova; Khamid U Khodjaniyazov; Kohei Torikai

doi:10.1021/acsomega.2c01688

Compositional Analysis and Potent Insecticidal Activity of Supercritical CO₂ Fluid Extracts of Alcea nudiflora L. Leaves

ACS Omega. 2022 Jun 2;7(23):19892-19897. doi: 10.1021/acsomega.2c01688. eCollection 2022 Jun 14.

Authors

Nazira K Khidyrova¹, Saida M Turaeva¹, Malohat J Rakhmatova¹, Khayrulla M Bobakulov^{1

2}, Shamansur S Sagdullaev¹, Rano P Zakirova¹, Khamid U Khodjaniyazov^{3

4}, Kohei Torikai^{4

5}

Affiliations

¹ S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, Uzbekistan.
² "Tashkent Institute of Irrigation and Agricultural Mechanization Engineers", National Research University, 39 Kori Niyoziy Str., Tashkent 100000, Uzbekistan.
³ A. S. Sadikov Institute of the Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, 83 Mirzo Ulugbek Str., Tashkent 100125, Uzbekistan.
⁴ Faculty of Chemistry, National University of Uzbekistan named after Mirzo Ulugbek, 4 University Str., Tashkent 100174, Uzbekistan.
⁵ Department of Chemistry, Faculty of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.

Abstract

To mitigate potentially severe food shortages due to the exponential growth of the global population, it is of paramount importance to improve the yield and quality of globally harvested food crops. As pest control contributes to both these aspects, the development of safe and effective pesticides is one of the main strategies pursued in this direction in the context of agricultural chemistry. During our investigation of natural pesticides, a supercritical CO₂ fluid extract of Alcea nudiflora L. was found to exert extremely potent insecticidal activity against aphids (Macrosiphum euphorbiae) and cowpea seed beetles (Callosobruchus maculatus) with LC₅₀ values of 0.03 mg/mL (24 h exposure, contact method). The facts that their insecticidal activity is in the most potent class among the essential oils known to date, and that the extract did not show any toxicity toward beneficial insects such as ladybugs (Coccinella magnifica) and European honeybees (Apis mellifera Linnaeus), indicate that this extract could be a good, natural, and safe new pesticide candidate. A compositional analysis of this extract was carried out using GC/MS.