Enhanced Antioxidant, Antiviral, and Anticancer Activities of the Extract of Fermented Egyptian Rice Bran Complexed with Hydroxypropyl-β-cyclodextrin

Sherif Ashraf Fahmy; Khaled A Nematallah; Noha Khalil Mahdy; Hesham I El-Askary; Meselhy Ragab Meselhy; Hassan Mohamed El-Said Azzazy

doi:10.1021/acsomega.2c01281

Enhanced Antioxidant, Antiviral, and Anticancer Activities of the Extract of Fermented Egyptian Rice Bran Complexed with Hydroxypropyl-β-cyclodextrin

ACS Omega. 2022 Jun 1;7(23):19545-19554. doi: 10.1021/acsomega.2c01281. eCollection 2022 Jun 14.

Authors

Sherif Ashraf Fahmy^{1

2}, Khaled A Nematallah³, Noha Khalil Mahdy¹, Hesham I El-Askary⁴, Meselhy Ragab Meselhy⁴, Hassan Mohamed El-Said Azzazy¹

Affiliations

¹ Department of Chemistry, School of Sciences and Engineering, The American University in Cairo, AUC Avenue, PO. Box 74, New Cairo, Cairo 11835, Egypt.
² School of Life and Medical Sciences, University of Hertfordshire, Global Academic Foundation, R5 New Garden City, New Cairo, Cairo 11835, Egypt.
³ Faculty of Pharmacy, Department of Pharmacognosy and Microbiology, The British University in Egypt, El Sherouk, Cairo 11837, Egypt.
⁴ Faculty of Pharmacy, Department of Pharmacognosy, Cairo University, Giza, Giza 12613, Egypt.

Abstract

Egyptian rice bran was fermented with baker's yeast, and released phenolics were extracted with aqueous methanol to give fermented rice bran extract (FRBE). The analysis of the FRBE with ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry revealed 21 compounds, mainly phenolic acids and flavonoids. The FRBE was then complexed with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) via noncovalent host-guest inclusion complexation using the thin-film hydration technique to improve the hydrophilicity and bioactivity of the FRBE. The formation of the inclusion complex was confirmed using HPLC, ¹H NMR, FT-IR, and a phase solubility study. In addition, the biological activities of the complex were investigated. The FRBE/HPβCD inclusion complex had more pronounced antioxidant, antiviral, and anticancer activities compared to free FRBE. These findings warrant the future investigation of potential medical applications of FRBE.