Ribosomal RNA Pseudouridylation: Will Newly Available Methods Finally Define the Contribution of This Modification to Human Ribosome Plasticity?

Chiara Barozzi; Federico Zacchini; Sidra Asghar; Lorenzo Montanaro

doi:10.3389/fgene.2022.920987

Ribosomal RNA Pseudouridylation: Will Newly Available Methods Finally Define the Contribution of This Modification to Human Ribosome Plasticity?

Front Genet. 2022 Jun 1:13:920987. doi: 10.3389/fgene.2022.920987. eCollection 2022.

Authors

Chiara Barozzi^{1

2}, Federico Zacchini^{1

2}, Sidra Asghar^{1

2}, Lorenzo Montanaro^{1

2

3}

Affiliations

¹ Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale (DIMES), Alma Mater Studiorum-Università di Bologna, Bologna, Italy.
² Centro di Ricerca Biomedica Applicata, CRBA, Universita di Bologna, Policlinico di Sant'Orsola, Bologna, Italy.
³ Departmental Program in Laboratory Medicine, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.

Abstract

In human rRNA, at least 104 specific uridine residues are modified to pseudouridine. Many of these pseudouridylation sites are located within functionally important ribosomal domains and can influence ribosomal functional features. Until recently, available methods failed to reliably quantify the level of modification at each specific rRNA site. Therefore, information obtained so far only partially explained the degree of regulation of pseudouridylation in different physiological and pathological conditions. In this focused review, we provide a summary of the methods that are now available for the study of rRNA pseudouridylation, discussing the perspectives that newly developed approaches are offering.

Keywords: cancer; pseudouridine detection; rRNA; ribosome; snoRNA.

Publication types

Review