Molecular characterization and expression of RPS23 and HPSE and their association with hematologic parameters in sheep

Jianghui Wang; Xiaojuan Wang; WeiminWang; Fadi Li; Deyin Zhang; Xiaolong Li; Yukun Zhang; Yuan Zhao; Liming Zhao; Dan Xu; Jiangbo Cheng; Wenxin Li; Bubo Zhou; Changchun Lin; Xiaobin Yang; Rui Zhai; Xiwen Zeng; Xiaoxue Zhang

doi:10.1016/j.gene.2022.146654

Molecular characterization and expression of RPS23 and HPSE and their association with hematologic parameters in sheep

Gene. 2022 Aug 30:837:146654. doi: 10.1016/j.gene.2022.146654. Epub 2022 Jun 16.

Authors

Jianghui Wang¹, Xiaojuan Wang², WeiminWang³, Fadi Li⁴, Deyin Zhang², Xiaolong Li¹, Yukun Zhang¹, Yuan Zhao¹, Liming Zhao¹, Dan Xu¹, Jiangbo Cheng¹, Wenxin Li¹, Bubo Zhou¹, Changchun Lin¹, Xiaobin Yang¹, Rui Zhai¹, Xiwen Zeng¹, Xiaoxue Zhang⁵

Affiliations

¹ College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
² The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China.
³ College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China; The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China.
⁴ The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China; Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, China.
⁵ College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China. Electronic address: zhangxx@gsau.edu.cn.

PMID: 35718240
DOI: 10.1016/j.gene.2022.146654

Abstract

Ribosomal protein S23 (RPS23) and Heparanase (HPSE) were located on chromosome 5 and chromosome 6, respectively, which play vital roles in protein synthesis and immunity. The objective of this study was to clone RPS23 and HPSE and to detect the expression levels of RPS23 and HPSE and the polymorphisms of RPS23 and HPSE associated with the hematologic parameters by using qRT-PCR, DNA sequencing and KASPar assay. The quantitative real-time PCR (RT-qPCR) showed that the two genes were expressed widely in the ten tissues of sheep. The expression levels of RPS23 and HPSE were the highest in lung and liver, respectively. The expression levels of RPS23 and HPSE in lung and liver increased from 0 to 3 months, decreased from 3 to 6 months, respectively. Furthermore, two mutations g.720 A > G and g.1077 G > A were detected in the RPS23 and HPSE, respectively, which were confirmed to be significantly associated with hematologic parameters. These results supported RPS23 g.720 A > G and HPSE g.1077 G > A as genetic markers of sheep.

Keywords: HPSE; Hematologic parameters; Immunity; RPS23; Sheep.

MeSH terms

Animals
Glucuronidase* / genetics
Liver Neoplasms* / genetics
Sheep / genetics

Substances

heparanase
Glucuronidase