Immunoglobulin heavy-chain loci in ancient allotetraploid goldfish

Linmei Han; Jihong Li; Wen Wang; Kaikun Luo; Mingli Chai; Caixia Xiang; Ziye Luo; Li Ren; Qianhong Gu; Min Tao; Chun Zhang; Jing Wang; Shaojun Liu

doi:10.1016/j.dci.2022.104476

Immunoglobulin heavy-chain loci in ancient allotetraploid goldfish

Dev Comp Immunol. 2022 Oct:135:104476. doi: 10.1016/j.dci.2022.104476. Epub 2022 Jun 16.

Authors

Linmei Han¹, Jihong Li¹, Wen Wang¹, Kaikun Luo¹, Mingli Chai¹, Caixia Xiang¹, Ziye Luo¹, Li Ren¹, Qianhong Gu¹, Min Tao¹, Chun Zhang¹, Jing Wang², Shaojun Liu³

Affiliations

¹ State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China.
² State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China. Electronic address: hwangjing0826@163.com.
³ State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China. Electronic address: lsj@hunnu.edu.cn.

PMID: 35718131
DOI: 10.1016/j.dci.2022.104476

Abstract

As an ancient allotetraploid species, goldfish (Carassius auratus) have two sets of subgenomes. In this study, immunoglobulin heavy-chain (IGH) genes were cloned from the red crucian carp (Carassius auratus red var.), and the corresponding loci were identified in the gynogenetic diploid red crucian carp (GRCC) genome as well as the genomes of three other goldfish strains (Wakin, G-12, and CaTCV-1). Examination showed that each goldfish strain possessed two sets of parallel IGH loci: a complete IGHA locus and a degenerated IGHB locus that was nearly 40 × smaller. In the IGHA locus, multiple τ chain loci were arranged in tandem between the μ&δ chain locus and the variable genes, but no τ-like genes were found in the IGHB locus.

Keywords: Allotetraploid; Carassius auratus; Chimeric gene; IGH loci; Immunoglobulin heavy-chain (IGH) genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carps* / genetics
Diploidy
Genome
Goldfish* / genetics
Immunoglobulins / genetics

Substances

Immunoglobulins