Tuberculosis Molecular Bacterial Load Assay Reveals Early Delayed Bacterial Killing in Patients With Relapse

Nyanda Elias Ntinginya; Abhishek Bakuli; Daniel Mapamba; Wilber Sabiiti; Gibson Kibiki; Lilian Tina Minja; Davis Kuchaka; Klaus Reither; Patrick Peter John Phillips; Martin Johan Boeree; Stephen H Gillespie; Michael Hoelscher; Norbert Heinrich; Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA) Consortium

doi:10.1093/cid/ciac445

Tuberculosis Molecular Bacterial Load Assay Reveals Early Delayed Bacterial Killing in Patients With Relapse

Clin Infect Dis. 2023 Feb 8;76(3):e990-e994. doi: 10.1093/cid/ciac445.

Authors

Nyanda Elias Ntinginya¹, Abhishek Bakuli^{2

3}, Daniel Mapamba¹, Wilber Sabiiti⁴, Gibson Kibiki⁵, Lilian Tina Minja⁶, Davis Kuchaka⁷, Klaus Reither^{6

8

9}, Patrick Peter John Phillips¹⁰, Martin Johan Boeree¹¹, Stephen H Gillespie⁴, Michael Hoelscher^{2

3}, Norbert Heinrich^{2

3}; Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA) Consortium

Affiliations

¹ National Institute for Medical Research-Mbeya Medical Research Centre, Mbeya, United Republic of Tanzania.
² Division of Infectious Diseases and Tropical Medicine, University Hospital, LMU Munich, Munich, Germany.
³ German Center for Infection Research, Munich Partner Site, Munich, Germany.
⁴ Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, United Kingdom.
⁵ East African Health Research Commission, Bujumbura, Burundi.
⁶ Ifakara Health Institute, Dar Es Salaam, United Republic of Tanzania.
⁷ Kilimanjaro Clinical Research Institute, Moshi, United Republic of Tanzania.
⁸ Swiss Tropical and Public Health Institute, Basel, Switzerland.
⁹ University of Basel, Basel, Switzerland.
¹⁰ Center for Tuberculosis, University of California, San Francisco, San Francisco, California, USA.
¹¹ Radboud Institute of Health Science, Radboud University Medical Center, Nijmegen, The Netherlands.

Abstract

Bacterial killing in patients with tuberculosis (TB) relapse was compared to that in patients achieving cure, measured by TB molecular bacterial load assay (TB-MBLA) or mycobacteria growth indicator tube (MGIT) time to positivity (TTP). TB-MBLA in 4 relapsed patients was significantly different compared to 132 cured patients after 2 weeks of treatment; MGIT TTP showed a significant difference from week 8.

Keywords: biomarker; clinical trial; relapse; tuberclosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Load
Humans
Mycobacterium tuberculosis*
Recurrence
Sputum / microbiology
Tuberculosis* / diagnosis
Tuberculosis* / microbiology

Grants and funding

UM1 AI068634/AI/NIAID NIH HHS/United States