ATP synthase subunit e is a shrimp growth-associated breeding marker

Yucheng Lu; Qingyun Liu; Zhiqi Huang; Xiuli Chen; Chunling Yang; Yueling Zhang; Yongzhen Zhao; Fan Wang

doi:10.1016/j.ygeno.2022.110410

ATP synthase subunit e is a shrimp growth-associated breeding marker

Genomics. 2022 Jul;114(4):110410. doi: 10.1016/j.ygeno.2022.110410. Epub 2022 Jun 15.

Authors

Yucheng Lu¹, Qingyun Liu², Zhiqi Huang¹, Xiuli Chen², Chunling Yang², Yueling Zhang³, Yongzhen Zhao⁴, Fan Wang⁵

Affiliations

¹ Department of Biology, College of Science, Shantou University, Shantou 515063, China.
² Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China.
³ Department of Biology, College of Science, Shantou University, Shantou 515063, China; Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; Shantou University-Universiti Malaysia Terengganu Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China.
⁴ Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China. Electronic address: Fisher1152002@gmail.com.
⁵ Department of Biology, College of Science, Shantou University, Shantou 515063, China; Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; Shantou University-Universiti Malaysia Terengganu Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China. Electronic address: wangfan@stu.edu.cn.

PMID: 35716822
DOI: 10.1016/j.ygeno.2022.110410

Abstract

Penaeus vannamei is one of the most popular aquaculture species in the world. This species is featured with its fast-growing and delicious taste, which drives people develop various strains. During this process identification of trait-associated markers could effectively increase breeding efficiency. Driven by this, we tried to screen fast-growing key regulators via a FACS-based high throughput method, in which 2-NBDG was applied as a fluorescent indicator for direct glucose uptake measurement. Totally six candidate genes were screened out followed by in vitro validation in 293T cells. After that, the correlation between these genes and shrimp growing was further verified in a hybrid lineage. The expression level of two genes including ATP synthase subunit e and inhibitor of apoptosis protein showed some correlation with shrimp growth speed. Furthermore, we tested these two candidate markers in various lineages and confirmed that ATP synthase subunit e could be a shrimp growth-associated breeding marker.

Keywords: ATP synthase subunit e; Growth-associated breeding marker; Penaeus vannamei.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate
Animals
Biomarkers
Breeding
Humans
Penaeidae* / genetics
Phenotype

Substances

Biomarkers
Adenosine Triphosphate