Cytotoxicity and osteogenic effects of aluminum ions

Kai Mizuno; Francesco Boschetto; Kenta Yamamoto; Taigi Honma; Nao Miyamoto; Tetsuya Adachi; Narisato Kanamura; Toshiro Yamamoto; Zhu Wenliang; Elia Marin; Giuseppe Pezzotti

doi:10.1016/j.jinorgbio.2022.111884

Cytotoxicity and osteogenic effects of aluminum ions

J Inorg Biochem. 2022 Sep:234:111884. doi: 10.1016/j.jinorgbio.2022.111884. Epub 2022 Jun 4.

Authors

Affiliations

¹ Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan.
² Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan; Department of Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, 465 Kajii-cho, Kyoto 602-8566, Japan.
³ Department of Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, 465 Kajii-cho, Kyoto 602-8566, Japan.
⁴ Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
⁵ Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan; Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan; Dipartimento Politecnico di Ingegneria e Architettura, University of Udine, 33100 Udine, Italy. Electronic address: elia-marin@kit.ac.jp.
⁶ Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan; Department of Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, 465 Kajii-cho, Kyoto 602-8566, Japan; Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan; Department of Orthopedic Surgery, Tokyo Medical University, 6-7-1 Nishi-Shinjuku, Shinjuku-ku, 160-0023 Tokyo, Japan; The Center for Advanced Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0854, Japan; Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.

PMID: 35716550
DOI: 10.1016/j.jinorgbio.2022.111884

Abstract

In this study, we monitored the effect of Al³⁺ ions on mesenchymal cells (KUSA-A1) and human fibroblasts (NHDF) by means of in vitro experiments by culturing the cells with addition of small concentrations of aluminum ions (i.e., 0.1, 1, 10, and 100 ppm). Bone formation test was then conducted using KUSA-A1. Small concentrations of aluminum ions delayed but did not completely inhibit cell proliferation. The amount of bone tissue decreased as the concentration of Al³⁺ increased and crystallinity changes were also detected by Raman spectroscopic experiments. Moreover, Al³⁺ ions greatly affected both structure and chemistry of bone tissues with mineral nodules becoming larger and atomic substitution of Ca with Al in bone tissue being more preponderant with increasing Al³⁺ concentration. Such effects in turn impaired the balance between mineral and collagen in the formed bone tissue.

Keywords: Aluminum; Aseptic loosening; Bone matrix; Hydroxyapatite; Metal ions; Mineralization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aluminum / toxicity
Collagen
Humans
Ions / pharmacology
Mesenchymal Stem Cells*
Osteogenesis*

Substances

Ions
Collagen
Aluminum