Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumonia

T Suzuki; M Saitou; Y Utano; K Utano; K Niitsuma

doi:10.1016/j.pulmoe.2022.04.003

Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumonia

Pulmonology. 2023 Sep-Oct;29(5):392-398. doi: 10.1016/j.pulmoe.2022.04.003. Epub 2022 Jun 15.

Authors

T Suzuki¹, M Saitou², Y Utano³, K Utano³, K Niitsuma²

Affiliations

¹ Department of Infectious Disease and Pulmonary Medicine, Aizu Medical Center, Fukushima Medical University, Fukushima, Japan; Department of General Internal Medicine, Saitama Medical University, Saitama, Japan. Electronic address: tomoko41@saitama-med.ac.jp.
² Department of Infectious Disease and Pulmonary Medicine, Aizu Medical Center, Fukushima Medical University, Fukushima, Japan.
³ Department of Radiology, Aizu Medical Center, Fukushima Medical University, Fukushima, Japan.

PMID: 35715334
DOI: 10.1016/j.pulmoe.2022.04.003

Abstract

Background and objective: There are currently no established markers for aspiration pneumonia. We hypothesized that bronchoalveolar lavage (BAL) amylase and pepsin might be candidate biomarkers for aspiration pneumonia.

Methods: This cross-sectional study reviewed consenting adults who underwent clinically-indicated bronchoscopy at Aizu Medical Center. BAL samples were obtained using standardized methods. Amylase levels were measured in our clinical laboratory, and pepsin levels were assessed by ELISA.

Results: Aspiration pneumonia was clinically diagnosed based on the guidelines of the Japanese Respiratory Society in 48 of the 327 participants. Median BAL salivary amylase and pepsin levels in this group were 702.0 U/L and 12.7 ng/ml respectively, which were significantly higher than in non-aspiration pneumonia patients. BAL amylase ≥204 U/L had 77.1% sensitivity and 84.2% specificity as a diagnostic index, and the area under the receiver operating characteristic (ROC) curve was 0.859 (95% confidence interval (CI), 0.803-0.915). Similarly, BAL pepsin levels of ≥7.45 ng/ml had 87.2% sensitivity and 59.9% specificity for identifying aspiration, and the area under the ROC curve was 0.757 (95% CI, 0.688-0.826). Multivariate regression demonstrated that BAL amylase ≥204 U/L and BAL pepsin ≥7.45 ng/ml were associated with significantly higher odds for aspiration pneumonia (odds ratio (OR) 10.0, 95% CI, 4.51-22.2, and OR 8.81 95% CI, 3.32-23.4, respectively). There were no significant associations between risk factors for aspiration pneumonia and BAL amylase and pepsin levels.

Conclusion: BAL amylase and pepsin might be useful biomarkers for suggesting aspiration pneumonia, and could be objective markers without relying on known risk factors for aspiration.

Keywords: Aspiration pneumonia; BAL amylase; BAL pepsin; Dripping of oral secretions; Microaspiration; Reflux of gastrointestinal contents.