HFAC Dose Repetition and Accumulation Leads to Progressively Longer Block Carryover Effect in Rat Sciatic Nerve

Adrien Rapeaux; Timothy G Constandinou

doi:10.3389/fnins.2022.852166

HFAC Dose Repetition and Accumulation Leads to Progressively Longer Block Carryover Effect in Rat Sciatic Nerve

Front Neurosci. 2022 May 27:16:852166. doi: 10.3389/fnins.2022.852166. eCollection 2022.

Authors

Adrien Rapeaux^{1

2}, Timothy G Constandinou^{1

2}

Affiliations

¹ Next Generation Neural Interfaces Lab, Centre for Bioinspired Technology, Department of Electrical and Electronic Engineering, Imperial College London, London, United Kingdom.
² Care Research and Technology Centre, UK Dementia Research Institute, London, United Kingdom.

Abstract

This paper describes high-frequency nerve block experiments carried out on rat sciatic nerves to measure the speed of recovery of A fibres from block carryover. Block carryover is the process by which nerve excitability remains suppressed temporarily after High Frequency Alternative (HFAC) block is turned off following its application. In this series of experiments 5 rat sciatic nerves were extracted and prepared for ex-vivo stimulation and recording in a specially designed perfusion chamber. For each nerve repeated HFAC block and concurrent stimulation trials were carried out to observe block carryover after signal shutoff. The nerve was allowed to recover fully between each trial. Time to recovery from block was measured by monitoring for when relative nerve activity returned to within 90% of baseline levels measured at the start of each trial. HFAC block carryover duration was found to be dependent on accumulated dose by statistical test for two different HFAC durations. The carryover property of HFAC block on A fibres could enable selective stimulation of autonomic nerve fibres such as C fibres for the duration of carryover. Block carryover is particularly relevant to potential chronic clinical applications of block as it reduces power requirements for stimulation to provide the blocking effect. This work characterizes this process toward the creation of a model describing its behavior.

Keywords: HFAC; alternating current; block; carryover; ex-vivo; high frequency; nerve; stimulation.