The Role of CD4+CD8+ T Cells in HIV Infection With Tuberculosis

Abstract

Background: Tuberculosis (TB) is an important opportunistic infection in acquired immunodeficiency diseases (AIDS). Although the frequency of CD4⁺CD8⁺ double-positive (DP) T cells has been observed to increase in pathological conditions, their role (phenotypic and functional) is poorly described, especially in human immunodeficiency virus (HIV) infection with TB (HIV/TB (HT) coinfection).

Methods: The percentage and phenotypic and functional properties of peripheral blood DP T cells in patients with HT coinfection in comparison to uninfected controls and to patients with HIV or TB mono-infection were analyzed by direct intracellular cytokine staining (ICS).

Results: Total and CD4^lowCD8^high DP T cells were significantly increased in patients with both HIV and TB mono-infection, especially in patients with HT coinfection. Compared with healthy controls (HCs), the percentage of DP T cells expressing chemokine receptor 5 (CCR5) in patients with HT coinfection was significantly higher. Compared with HCs and patients with TB, a lower percentage of tumor necrosis factor α (TNF-α) secreting DP T cells and a higher percentage of granzyme A-secreting DP T cells were observed in patients with HIV mono-infection and HT coinfection, respectively. In addition, DP T cells expressed more cytolytic markers (granzyme A and perforin) than CD4⁺ T cells, but similarly to CD8⁺ T cells in patients with HT coinfection.

Conclusions: Our data suggested that HT coinfection resulted in a marked increase in DP T cells, especially the CD4^lowCD8^high subpopulation. DP T cells may be susceptible to HT coinfection, and have the same cytotoxic function as CD8⁺ T cells.

Keywords: CCR5; HIV; double positive (DP) T cells; granzyme A; tuberculosis (TB).