Phytochemical Profiling, Antioxidant Activity, and In Silico Analyses of Sterculia villosa and Vernonia patula

Chadni Lyzu; Saikat Mitra; Kahkashan Perveen; Zidan Khan; Abu Montakim Tareq; Najat A Bukhari; Fohad Mabood Husain; Evena Parvin Lipy; Dipa Islam; Mahmuda Hakim; Talha Bin Emran; Marjan Ganjali Dashti

doi:10.1155/2022/3190496

Phytochemical Profiling, Antioxidant Activity, and In Silico Analyses of Sterculia villosa and Vernonia patula

Evid Based Complement Alternat Med. 2022 Jun 6:2022:3190496. doi: 10.1155/2022/3190496. eCollection 2022.

Authors

Affiliations

¹ Biomedical and Toxicological Research Institute, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka 1205, Bangladesh.
² Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
³ Department of Botany & Microbiology, College of Science, King Saud University, Riyadh-11495, Saudi Arabia.
⁴ Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh.
⁵ Department o Food Science and Nutrition, College of Food and Agriculture, King Saud University, Riyadh 11421, Saudi Arabia.
⁶ Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh.
⁷ Department of Pharmacy, Faculty of Allied Health Sciences, Daffodil International University, Dhaka 1207, Bangladesh.
⁸ Department of Biological Sciences, University of Texas at Dallas, 800 W Campbell Road, Richardson 75080, TX, USA.

Abstract

Our study aims to evaluate the chemical profiles and antioxidant activities of a methanolic extract of Sterculia villosa bark (MESV) and a methanolic extract of the Vernonia patula whole plant (MEVP). The chemical profiling of MESV and MEVP was performed via gas chromatography-mass spectrometry (GC-MS), which identified 52 and 33 chemical compounds, respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay indicated that both MESV and MEVP displayed concentration-dependent scavenging activities, and half-maximal inhibitory concentration (IC₅₀) values for MEVP, MESV, and ascorbic acid were 305.30, 555.44, and 36.32 μg/mL, respectively. The total flavonoid content (TFC) and total phenolic content (TPC) of MESV were 81.44 ± 2.70 mg quercetin equivalents (QE)/g dry extract and 62.58 ± 1.93 mg gallic acid equivalent (GAE)/g dry extract, whereas these values for MEVP were 291.31 ± 6.61 mg QE/g dry extract and 58.99 ± 3.16 mg GAE/g dry extract, respectively. Molecular docking studies were also evaluated, and absorption, distribution, metabolism, and excretion (ADME) and toxicological properties were assessed. Therefore, these two plants, S. villosa and V. patula, showed potential options for further advanced studies into oxidative stress.