Dual-Driven Hemostats Featured with Puncturing Erythrocytes for Severe Bleeding in Complex Wounds

Haoyu Qiu; Guangqian Lan; Weiwei Ding; Xinyu Wang; Wenyi Wang; Dahua Shou; Fei Lu; Enling Hu; Kun Yu; Songmin Shang; Ruiqi Xie

doi:10.34133/2022/9762746

Dual-Driven Hemostats Featured with Puncturing Erythrocytes for Severe Bleeding in Complex Wounds

Research (Wash D C). 2022 May 31:2022:9762746. doi: 10.34133/2022/9762746. eCollection 2022.

Authors

Haoyu Qiu¹, Guangqian Lan^{1

2}, Weiwei Ding³, Xinyu Wang³, Wenyi Wang⁴, Dahua Shou⁴, Fei Lu^{1

2}, Enling Hu^{1

2

4}, Kun Yu^{1

2}, Songmin Shang⁴, Ruiqi Xie^{1

2

4}

Affiliations

¹ State Key Laboratory of Silkworm Genome Biology, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China.
² Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
³ Division of Trauma and Surgical Intensive Care Unit, Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002 Jiangsu Province, China.
⁴ Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Kowloon, Hong Kong.

Abstract

Achieving rapid hemostasis in complex and deep wounds with secluded hemorrhagic sites is still a challenge because of the difficulty in delivering hemostats to these sites. In this study, a Janus particle, SEC-Fe@CaT with dual-driven forces, bubble-driving, and magnetic field- (MF-) mediated driving, was prepared via in situ loading of Fe₃O₄ on a sunflower sporopollenin exine capsule (SEC), and followed by growth of flower-shaped CaCO₃ clusters. The bubble-driving forces enabled SEC-Fe@CaT to self-diffuse in the blood to eliminate agglomeration, and the MF-mediated driving force facilitated the SEC-Fe@CaT countercurrent against blood to access deep bleeding sites in the wounds. During the movement in blood flow, the meteor hammer-like SEC from SEC-Fe@CaT can puncture red blood cells (RBCs) to release procoagulants, thus promoting activation of platelet and rapid hemostasis. Animal tests suggested that SEC-Fe@CaT stopped bleeding in as short as 30 and 45 s in femoral artery and liver hemorrhage models, respectively. In contrast, the similar commercial product Celox™ required approximately 70 s to stop the bleeding in both bleeding modes. This study demonstrates a new hemostat platform for rapid hemostasis in deep and complex wounds. It was the first attempt integrating geometric structure of sunflower pollen with dual-driven movement in hemostasis.