Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage

Adele Alagia; Ruth F Ketley; Monika Gullerova

doi:10.1007/978-1-0716-2477-7_19

Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage

Methods Mol Biol. 2022:2528:289-303. doi: 10.1007/978-1-0716-2477-7_19.

Authors

Adele Alagia¹, Ruth F Ketley¹, Monika Gullerova²

Affiliations

¹ Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
² Sir William Dunn School of Pathology, University of Oxford, Oxford, UK. monika.gullerova@path.ox.ac.uk.

PMID: 35704199
DOI: 10.1007/978-1-0716-2477-7_19

Abstract

In situ Proximity Ligation Assay (PLA ) can be used to detect the close proximity (less than ~40 nm) of two biological molecules of interest in cells. Here we report the application of this method for the specific detection of R-loop interacting proteins and RNA modifications in close proximity to R-loops in non-damage and ionizing radiation (IR) induced DNA damage conditions.

Keywords: DNA Damage; Double Strand breaks; Proximity Ligation Assay; R-loops; RNA modification; RNA:DNA hybrids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Damage*
Proteins
R-Loop Structures*
Radiation, Ionizing

Substances

Proteins

Grants and funding

BVR01170 /CRUK_/Cancer Research UK/United Kingdom