Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin

Peiyan Ni; Chuqing Zhou; Yajing Meng; Rui Xue; Xia Yang; Lei Li; Liansheng Zhao; Jinxue Wei; Rongjun Ni; Yingcheng Wang; Xiaohong Ma; Wanjun Guo; Qiang Wang; Tao Li

doi:10.1016/j.scr.2022.102832

Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin

Stem Cell Res. 2022 Aug:63:102832. doi: 10.1016/j.scr.2022.102832. Epub 2022 Jun 7.

Authors

Peiyan Ni¹, Chuqing Zhou¹, Yajing Meng¹, Rui Xue¹, Xia Yang¹, Lei Li¹, Liansheng Zhao¹, Jinxue Wei¹, Rongjun Ni¹, Yingcheng Wang¹, Xiaohong Ma¹, Wanjun Guo¹, Qiang Wang¹, Tao Li²

Affiliations

¹ The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China.
² Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, Zhejiang, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address: litaozjusc@zju.edu.cn.

PMID: 35700633
DOI: 10.1016/j.scr.2022.102832

Abstract

Schizophrenia (SCZ) and bipolar disorder (BD) are debilitating neurodevelopmental disorders with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by three females. An adolescent female was clinically diagnosed as first-episode SCZ. One of her cousins was clinically diagnosed as BD and another one was unaffected control. Induced pluripotent stem cells (iPSCs) were established with reprograming factors Oct4, Sox2, Nanog, Lin28, c-myc, Klf4, and SV40LT. All lines presented normal karyotype and highly expressed pluripotency markers in vitro. All iPSCs were capable to differentiate into derivatives of three germ layers in vivo.

Keywords: Bipolar disorder (BD); Induced pluripotent stem cells (iPSCs); Psychiatric disorders; Schizophrenia (SCZ).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Bipolar Disorder*
Cell Differentiation
Family
Female
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Schizophrenia*