A multifunctional chitosan hydrogel dressing for liver hemostasis and infected wound healing

Abstract

For the treatment of infected bleeding wounds, we compounded methacrylate anhydride dopamine (DAMA) and Zn-doped whitlockite nanoparticles (Zn-nWH) into methacrylate anhydride quaternized chitosan (QCSMA) to obtain a multifunctional hydrogel dressing (QCSMA/DAMA/Zn-nWH) with hemostasis, disinfection and wound healing promotion. QCSMA/DAMA/Zn-nWH exhibited good adhesion (0.031 MPa) and DPPH scavenging ability (94%), favorable biocompatibility (hemolysis ratio < 2%, no cytotoxicity), and showed a low BCI value (< 13%) in vitro coagulation test and could activate coagulation pathway. In addition, QCSMA/DAMA/Zn-nWH had excellent hemostatic effect (129 ± 22 s, 27 ± 5 mg) in vivo compared with the control (571 ± 15 s, 147 ± 31 mg) and CCS (354 ± 27 s, 110 ± 46 mg). Meanwhile, QCSMA/DAMA/Zn-nWH showed excellent antibacterial properties (> 90% against S. aureus and E. coli) and could promote collagen deposition, reduce inflammatory expression and promote wound healing. All results indicate that these multifunctional hydrogel dressings have great potential in clinical hemostasis and anti-infection healing.

Keywords: Antibacterial; Calcium hydroxide (PubChem CID: 14777); Chitosan; Chitosan (PubChem CID: 71853); Dopamine hydrochloride (PubChem CID: 65340); Glycidyltrimethylammonium chloride (PubChem CID: 18205); Hemostasis; Lithium Phenyl (2,4,6-trimethylbenzoyl) phosphonate (PubChem CID: 68384915); Magnesium hydroxide (PubChem CID: 14791); Methacrylate anhydride (PubChem CID: 12974); Phosphoric acid (PubChem CID: 1004); Wound healing; Zinc hydroxide (PubChem CID: 9812759).