The antidiabetic effect and potential mechanisms of the polysaccharides from the fruits of Lycium barbarum L. (LBPs) by the mouse model of high-fat diet/streptozotocin-induced diabetes were investigated. Six-week oral administration of LBPs (200 mg/kg/day) resulted in improvement in the levels of fasting blood glucose (13.51% decrease) and glycated hemoglobin and β-cell function in diabetic mice, and simultaneously induced a 3.3-fold increment in one taxon belonging to genus Allobaculum in gut bacterial community. The experiments of fecal microbiota transplantation and antibiotics treatment confirmed that the LBPs-mediated gut microbiota participated in the glycemic control of the diabetes management. Moreover, LBPs intervention guarded the intestinal barrier function via upregulating the expression of zonula occludens 1 both in vivo (analyzing the gut permeability in diabetic mice) and in vitro (using intestinal-like Caco-2/RAW264.7 cells co-culture inflammation model). Collectively, our study showed that LBPs could confer anti-diabetic effect through modifying gut microbiota and intestinal barrier.
Keywords: 2-Ethylbutyric acid (PubChem CID: 6915); Acetic acid (PubChem CID: 176); Ampicillin (PubChem CID: 6249); Barrier function; Gut microbiota; Hypoglycemic activity; Lycium barbarum L.; Metronidazol (PubChem CID: 4173); Neomycin sulfate (PubChem CID: 197162); Polysaccharides; Propionic acid (PubChem CID: 1032); Streptozotocin (PubChem CID: 29327); Vancomycin (PubChem CID: 14969); n-Butyric acid (PubChem CID: 264); n-Valeric acid (PubChem CID: 7991).
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