The structure and flocculation characteristics of a novel exopolysaccharide from a Paenibacillus isolate

Shijunyin Chen; Rui Cheng; Xiaodong Xu; Changchang Kong; Lei Wang; Renjie Fu; Jing Li; Shiming Wang; Jianfa Zhang

doi:10.1016/j.carbpol.2022.119561

The structure and flocculation characteristics of a novel exopolysaccharide from a Paenibacillus isolate

Carbohydr Polym. 2022 Sep 1:291:119561. doi: 10.1016/j.carbpol.2022.119561. Epub 2022 May 6.

Authors

Shijunyin Chen¹, Rui Cheng², Xiaodong Xu³, Changchang Kong⁴, Lei Wang⁵, Renjie Fu⁶, Jing Li⁷, Shiming Wang⁸, Jianfa Zhang⁹

Affiliations

¹ Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: chensh@njust.edu.cn.
² Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: chengrui@njust.edu.cn.
³ Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: xiaodong-xu@njust.edu.cn.
⁴ Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: kongchang@njust.edu.cn.
⁵ Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: 315102000263@njust.edu.cn.
⁶ Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China.
⁷ Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: jingli.seb@njust.edu.cn.
⁸ Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: bioeng@njust.edu.cn.
⁹ Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing 210094, China. Electronic address: jfzhang@njust.edu.cn.

PMID: 35698387
DOI: 10.1016/j.carbpol.2022.119561

Abstract

Bioflocculants like polysaccharides are excellent alternatives to harmful synthetic flocculants and usability under stricter levels of ecosystem protection. In this study, an exopolysaccharide producing strain Paenibacillus sp. M21629 was isolated. The exopolysaccharide was abundant in carboxylic groups and named floccuronic acid with a high weight average molecular weight of 2.38 × 10⁸ Da. The hexasaccharide repeating units of floccuronic acid were →3)-α-D-Manp-(1 → 3)-4-Suc-β-D-Manp-(1 → 3)-β-D-Glcp-(1 → 4)-β-D-GlcpA-(1 → 4)[4,6-Pyr-β-D-Galp-(1 → 3)]-β-D-Galp-(1→. The floccuronic acid at 0.5-1 ppm showed high turbidity removal efficiency in kaolin suspension (99.8%), coal wastewater (98.8%), and high-turbidity drinking water (89.2%). The flocculation ability of floccuronic acid depended on the instability of solid particles. Owing to these encouraging results, floccuronic acid is expected to be a useful bioflocculant applied in wastewater treatment and the utilization of water resources.

Keywords: Bioflocculant; Exopolysaccharide; Paenibacillus sp. M21629.

MeSH terms

Acids
Ecosystem
Flocculation
Paenibacillus*
Wastewater / chemistry
Water Purification* / methods

Substances

Acids
Waste Water