Polysaccharide is one of the main active components of Polygonatum sibiricum. For this study, P. sibiricum polysaccharides (PSP) were obtained through purification using DEAE-Cellulose52 and Sephacryl G-150 column chromatography. The obtained samples were named PSP1, PSP2 and PSP3. The PSP1 sample was found to have the highest content and the best solubility, and a subsequent. So, its structure and characterization were analyzed. The main sugar residue linkages were found to be → 1)-β-D-Fruf-(2 → 1)-β-D-Fruf-(2 → 1), 1 → -β-D-Fruf-(2 → 6)α-D-Glcp (1→, →4)-β-D-Manp-(1 → 4)-β-D-Manp-(1→ and →6)-β-D-Glcp-(1 → 4)-β-D-Manp-(1→ link existed. Branch chain analysis indicated →1,6)-β-D-Fruf-(2→, β-D-Fruf-(2→, →1,6)-β-D-Fruf-(2→, →6)-β-D- Fruf-(2→ link existed, and the link site was at position C-6. In vitro antioxidant activity tests showed that PSP1 had a certain scavenging effect on DPPH, hydroxyl radical, superoxide anion radical and a particular effect on the chelating ability of ferrous. This suggested that P. sibiricum polysaccharides may be a potential antioxidant.
Keywords: Antioxidant activity; Polygonatum sibiricum polysaccharides; Structure characterization.
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