High-throughput characterization of the filamentous cyanobacterium Anabaena sp. using flow cytometry

Philipp Doppler; Ricarda Kriechbaum; Oliver Spadiut

doi:10.1016/j.mimet.2022.106510

High-throughput characterization of the filamentous cyanobacterium Anabaena sp. using flow cytometry

J Microbiol Methods. 2022 Aug:199:106510. doi: 10.1016/j.mimet.2022.106510. Epub 2022 Jun 11.

Authors

Philipp Doppler¹, Ricarda Kriechbaum², Oliver Spadiut³

Affiliations

¹ Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: philipp.doppler@tuwien.ac.at.
² Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: ricarda.kriechbaum@tuwien.ac.at.
³ Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria. Electronic address: oliver.spadiut@tuwien.ac.at.

PMID: 35697185
DOI: 10.1016/j.mimet.2022.106510

Abstract

In stirred-tank photobioreactors agitation causes fragmentation of filamentous cyanobacteria. Here, we introduce a flow cytometric approach for high-throughput measurements of trichome dimensions, heterocysts and metabolic activity of Anabaena sp. cultures. The longest characterized trichome had 1135 μm chain length. This technology could potentially be used for monitoring and control purposes.

Keywords: Anabaena sp. PCC7120; Chlorophyll fluorescence; Heterocysts; Stirred-tank photobioreactor; Trichomes; Viability staining.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anabaena* / metabolism
Bacterial Proteins / genetics
Flow Cytometry
Gene Expression Regulation, Bacterial

Substances

Bacterial Proteins