Mesonosides A-H, primeverose derivatives from Mesona procumbens suppress adipogenesis by downregulating PPARγ and C/EBPα in 3T3-L1 cells

Hung-Tse Huang; Chia-Ching Liaw; Chun-Tang Chiou; Kung-Ta Lee; Yao-Haur Kuo

doi:10.38212/2224-6614.3365

Mesonosides A-H, primeverose derivatives from Mesona procumbens suppress adipogenesis by downregulating PPARγ and C/EBPα in 3T3-L1 cells

J Food Drug Anal. 2021 Sep 15;29(3):448-467. doi: 10.38212/2224-6614.3365.

Authors

Hung-Tse Huang^{1

2}, Chia-Ching Liaw^{2

3}, Chun-Tang Chiou², Kung-Ta Lee¹, Yao-Haur Kuo^{2

4}

Affiliations

¹ Department of Biochemical Science and Technology, National Taiwan University, Taipei, 10617, Taiwan.
² Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan.
³ Department of Biochemical Science and Technology, National Chiayi University, Chiayi, 60004, Taiwan.
⁴ Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, 40447, Taiwan.

Abstract

Obesity is becoming a worldwide epidemic, especially in industrialized countries. We hereby report a methanolic extract of Mesona procumbens (known as Hsian-tsao in Taiwan) significantly inhibits lipid accumulation in 3T3-L1 adipocytes, and eight new primeverose derivatives, mesonosides A-H (1-8), were isolated from the methanolic extract of M. procumbens. Structural elucidation of 1-8 was established by spectroscopic methods, especially 2D NMR techniques (¹H-¹H COSY, HSQC, HMBC, and NOESY) and HRESIMS. Anti-obesity evaluation revealed that isolates 1-5, 7, and 8 showed inhibitory effects on lipid accumulation and protein levels of adipogenic transcription factor, PPARγ and C/EBPα in 3T3-L1 cells. Our study suggests that M. procumbens extract including new primeverose isolates may be potentially used as a natural source to ameliorate fat accumulation and even obesity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3-L1 Cells
Adipogenesis*
Animals
CCAAT-Enhancer-Binding Protein-alpha / genetics
CCAAT-Enhancer-Binding Protein-alpha / metabolism
CCAAT-Enhancer-Binding Protein-alpha / pharmacology
Lamiaceae* / metabolism
Lipids
Mice
Obesity / metabolism
PPAR gamma / genetics
PPAR gamma / metabolism
PPAR gamma / pharmacology
Plant Extracts / pharmacology

Substances

CCAAT-Enhancer-Binding Protein-alpha
Lipids
PPAR gamma
Plant Extracts

Grants and funding

The authors gratefully acknowledge the support of the Ministry of Science and Technology of Taiwan (MOST 107-2320-B-077-003-MY3) for funding this work.