Lucigenin-pyrogallol chemiluminescence for the multiple detection of pyrogallol, cobalt ion, and tyrosinase

J Food Drug Anal. 2021 Sep 15;29(3):510-520. doi: 10.38212/2224-6614.3361.

Abstract

Developing new, cheap, sensitive and selective chemiluminescence (CL) systems with multiple detection properties is still a big challenge for biological and environmental applications. Here, we report a new CL system having multiple detection applications (environmental and biological). The developed lucigenin-pyrogallol system gave an enhancement (190 times) over the conventional lucigenin-H2O2 CL system. Cobalt ion and tyrosinase can inhibit lucigenin-PG CL intensity. Based on these phenomena, we have developed new CL methods for the detection of pyrogallol (LOD = 0.94 μM), lucigenin (LOD = 0.42 μM), cobalt (LOD = 68 nM), and tyrosinase (LOD = 1.8 μg/mL). Furthermore, the developed system gave excellent recoveries in real samples within the range of 93.59-103.12% for the assay of pyrogallol, Co2+ and tyrosinase in coffee, lake water, and human plasma, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acridines
  • Cobalt
  • Humans
  • Hydrogen Peroxide
  • Luminescence*
  • Luminescent Measurements / methods
  • Monophenol Monooxygenase
  • Pyrogallol*

Substances

  • Acridines
  • Pyrogallol
  • 10,10'-dimethyl-9,9'-biacridinium
  • Cobalt
  • Hydrogen Peroxide
  • Monophenol Monooxygenase

Grants and funding

This project was kindly supported by the National Natural Science Foundation of China [No. 21804127, and 21874126], Department of Science and Technology of Jilin Province (No. 20200703024ZP), the National Key Research and Development Program of China [No. 2016YFA0201300], and the Chinese Academy of Sciences (CAS)-the Academy of Sciences for the Developing World (TWAS) President’s Fellowship Programme.