Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein System for Resistance Against Plant Viruses: Applications and Perspectives

Fredy D A Silva; Elizabeth P B Fontes

doi:10.3389/fpls.2022.904829

Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein System for Resistance Against Plant Viruses: Applications and Perspectives

Front Plant Sci. 2022 May 26:13:904829. doi: 10.3389/fpls.2022.904829. eCollection 2022.

Authors

Fredy D A Silva¹, Elizabeth P B Fontes¹

Affiliation

¹ Department of Biochemistry and Molecular Biology/Bioagro, National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Viçosa, Viçosa, Brazil.

Abstract

Different genome editing approaches have been used to engineer resistance against plant viruses. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas; CRISPR/Cas) systems to create pinpoint genetic mutations have emerged as a powerful tool for molecular engineering of plant immunity and increasing resistance against plant viruses. This review presents (i) recent advances in engineering resistance against plant viruses by CRISPR/Cas and (ii) an overview of the potential host factors as targets for the CRISPR/Cas system-mediated broad-range resistance and immunity. Applications, challenges, and perspectives in enabling the CRISPR/Cas system for crop protection are also outlined.

Keywords: CRISPR/Cas; genome editing; plant antiviral immunity; resistance to viruses; susceptibility genes; virus-host interactions.

Publication types

Review