The new X-ray/visible microscopy MAXWELL technique for fast three-dimensional nanoimaging with isotropic resolution

Yoshiki Kohmura; Shun-Min Yang; Hsiang-Hsin Chen; Hidekazu Takano; Chia-Ju Chang; Ya-Sian Wang; Tsung-Tse Lee; Ching-Yu Chiu; Kai-En Yang; Yu-Ting Chien; Huan-Ming Hu; Tzu-Ling Su; Cyril Petibois; Yi-Yun Chen; Cheng-Huan Hsu; Peilin Chen; Dueng-Yuan Hueng; Shean-Jen Chen; Chi Lin Yang; An-Lun Chin; Chian-Ming Low; Francis Chee Kuan Tan; Alvin Teo; Eng Soon Tok; Xu Xiang Cai; Hong-Ming Lin; John Boeckl; Anton P Stampfl; Jumpei Yamada; Satoshi Matsuyama; Tetsuya Ishikawa; Giorgio Margaritondo; Ann-Shyn Chiang; Yeukuang Hwu

doi:10.1038/s41598-022-13377-w

The new X-ray/visible microscopy MAXWELL technique for fast three-dimensional nanoimaging with isotropic resolution

Sci Rep. 2022 Jun 11;12(1):9668. doi: 10.1038/s41598-022-13377-w.

Authors

Yoshiki Kohmura^#¹, Shun-Min Yang^#², Hsiang-Hsin Chen^#², Hidekazu Takano³, Chia-Ju Chang², Ya-Sian Wang², Tsung-Tse Lee², Ching-Yu Chiu², Kai-En Yang², Yu-Ting Chien², Huan-Ming Hu², Tzu-Ling Su², Cyril Petibois², Yi-Yun Chen², Cheng-Huan Hsu², Peilin Chen^{2

4}, Dueng-Yuan Hueng⁵, Shean-Jen Chen⁶, Chi Lin Yang⁷, An-Lun Chin⁷, Chian-Ming Low^{8

9}, Francis Chee Kuan Tan⁹, Alvin Teo¹⁰, Eng Soon Tok¹¹, Xu Xiang Cai¹², Hong-Ming Lin¹², John Boeckl¹³, Anton P Stampfl¹⁴, Jumpei Yamada³, Satoshi Matsuyama¹⁵, Tetsuya Ishikawa³, Giorgio Margaritondo¹⁶, Ann-Shyn Chiang^{2

7}, Yeukuang Hwu^{17

18}

Affiliations

¹ RIKEN/SPring-8 Center, Hyogo, 679-5148, Japan. yoshiki.kohmura@riken.jp.
² Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
³ RIKEN/SPring-8 Center, Hyogo, 679-5148, Japan.
⁴ Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
⁵ Department of Surgery, School of Medicine, National Defense Medical Center, Taipei, Taiwan.
⁶ College of Photonics, National Yang Ming Chiao Tung University, Tainan, Taiwan.
⁷ Brain Research Center, National Tsing Hua University, Hsinchu, Taiwan.
⁸ Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
⁹ Department of Anaesthesia, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
¹⁰ School of Chemical and Life Sciences, Nanyang Polytechnic, Singapore, Singapore.
¹¹ ƐMaGIC-Lab, Department of Physics, National University of Singapore, Singapore, Singapore.
¹² Mechanical and Materials Department, Tatung University, Taipei, Taiwan.
¹³ US Air Force Research Laboratory, Materials and Manufacturing Directorate, WPAFB, Fairborn, OH, 43455, USA.
¹⁴ Australian Nuclear Science and Technology Organisation, Sydney, NSW, 2234, Australia.
¹⁵ Department of Materials Physics, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8603, Japan.
¹⁶ Ecole Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.
¹⁷ Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan. phhwu@sinica.edu.tw.
¹⁸ Brain Research Center, National Tsing Hua University, Hsinchu, Taiwan. phhwu@sinica.edu.tw.

^# Contributed equally.

Abstract

Microscopy by Achromatic X-rays With Emission of Laminar Light (MAXWELL) is a new X-ray/visible technique with attractive characteristics including isotropic resolution in all directions, large-volume imaging and high throughput. An ultrathin, laminar X-ray beam produced by a Wolter type I mirror irradiates the sample stimulating the emission of visible light by scintillating nanoparticles, captured by an optical system. Three-dimensional (3D) images are obtained by scanning the specimen with respect to the laminar beam. We implemented and tested the technique with a high-brightness undulator at SPring-8, demonstrating its validity for a variety of specimens. This work was performed under the Synchrotrons for Neuroscience-an Asia-Pacific Strategic Enterprise (SYNAPSE) collaboration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Imaging, Three-Dimensional
Light
Microscopy* / methods
Synchrotrons*
Tomography, X-Ray Computed / methods
X-Rays