Inhibition of nitrite in prepared dish of Brassica chinensis L. during storage via non-extractable phenols in hawthorn pomace: A comparison of different extraction methods

Abstract

The objective of this study was to investigate whether non-extractable phenols (NEP) prepared by acid, enzymatic and alkaline hydrolysis in hawthorn pomace could reduce the nitrite content in prepared vegetable dishes (PVDs), analyzed through ultraviolet spectrophotometry and high performance liquid chromatography. The results showed that on the seventh day of storage, compared with the control group, the nitrite content of the samples added with acid, enzymatic and alkaline hydrolyzed NEP decreased by 40%, 28% and 19%, respectively, depending on different contents and chemical compositions of the recovered NEP. The nitrite reduction caused by NEP was mainly attributed to the growth inhibition of microorganisms producing nitrite (e.g., Escherichia coli and Pseudomonas aeruginosa) and the direct scavenging effect on nitrite, rather than affecting the activities of nitrate reductases and nitrite reductases in plant tissues. Use of hawthorn pomace is potentially a promising option to reduce nitrite in PVDs.

Keywords: Extraction methods; Hawthorn pomace; Nitrite; Non-extractable phenols; Prepared vegetable dishes.