3D Printed Polylactic Acid Well-Plate for Multi-enzyme Immobilization

Elena Gkantzou; Anastasia Skonta; Andreas-Georgios Vasios; Haralambos Stamatis

doi:10.1007/978-1-0716-2269-8_10

3D Printed Polylactic Acid Well-Plate for Multi-enzyme Immobilization

Methods Mol Biol. 2022:2487:163-175. doi: 10.1007/978-1-0716-2269-8_10.

Authors

Elena Gkantzou¹, Anastasia Skonta², Andreas-Georgios Vasios², Haralambos Stamatis³

Affiliations

¹ Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece. e.gkantzou@uoi.gr.
² Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece.
³ Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece. hstamati@uoi.gr.

PMID: 35687235
DOI: 10.1007/978-1-0716-2269-8_10

Abstract

3D printing is lately utilized in biological sciences under the scope to develop customized scaffolds that will host biomolecules, either whole cells or parts of them, like enzymes. In the present work, we present a protocol to modify the surface of 3D printed polylactic acid (PLA) well-plates with the aim to co-immobilize multiple enzymes that will perform cascade reactions. Detailed steps to design and print the final models are described. The developed protocol for surface modification is based on coating with chitosan biopolymer and covalent immobilization of the enzymes β-glucosidase, glucose oxidase, and peroxidase via glutaraldehyde cross-linking. Enzymatic activity measurements indicative of the catalytic performance of the system are also presented.

Keywords: 3D design; 3D printing; Chitosan; Multi-enzyme immobilization; PLA well-plate; Polylactic acid; Surface modification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chitosan*
Enzymes, Immobilized
Polyesters*
Printing, Three-Dimensional

Substances

Enzymes, Immobilized
Polyesters
poly(lactide)
Chitosan