Objective: To investigate the anti-oxidative effect of ethyl pyruvate (EP) and taurine (TAU) on the quality of red blood cells stored at 4±2 ℃, hemolysis, energy metabolism and lipid peroxidation of the red blood cells in the preservation solution were studied at different intervals.
Methods: At 4±2 ℃, the deleukocyte red blood cells were stored in the citrate-phosphate-dextrosesaline-adenine-1 (CPDA-1) preservation (control group), preservation solution with EP (EP-AS), and TAU (TAU-AS) for long-term preservation. The enzyme-linked immunoassay and automatic blood cell analyzer were used to detect hemolysis and erythrocyte parameters. Adenine nucleoside triphosphate (ATP), glycerol 2,3-diphosphate (2,3-DPG) and malondialdehyde (MDA) kits were used to test the ATP, 2,3-DPG and MDA concentration.
Results: During the preservation, the rate of red blood cell hemolysis in EP-AS and TAU-AS groups were significantly lower than that in CPDA-1 group (P<0.01). The MCV of EP-AS group was increased with the preservation time (r=0.71), while the MCV of the TAU-AS group was significantly lower than that in the other two groups (P<0.05). The concentration of ATP and MDA in EP-AS and TAU-AS groups were significantly higher than that in CPDA-1 group at the 14th day (P<0.01). The concentrations of 2,3-DPG in the EP-AS and TAU-AS groups were significantly higher than that in the CPDA-1 group from the 7th day (P<0.01).
Conclusion: EP and TAU can significantly reduce the red blood cell hemolysis rate, inhibit the lipid peroxidation level of red blood cells, and improve the energy metabolism of red blood cells during storage. The mechanism of EP and TAU may be related to their antioxidation and membrane protection effect, so as to improve the red blood cell quality and extend the preservation time.
题目: 丙酮酸乙酯、牛磺酸的抗氧化作用对红细胞4 ℃保存的影响.
目的: 研究丙酮酸乙酯(EP)或牛磺酸(TAU)的保存液对红细胞4±2 ℃保存时溶血、能量代谢和脂质过氧化等指标随保存时间延长的变化，探讨上述物质对红细胞4±2 ℃保存质量的影响.
方法: 在4±2 ℃，将去白细胞浓缩红细胞分别保存在CPDA-1（对照组）以及添加EP (EP-AS组)或TAU (TAU-AS组)的保存液中，进行长期保存。应用酶联免疫检测仪和全自动血细胞分析仪定期对各组保存红细胞进行溶血和红细胞参数测定；采用腺嘌呤核苷三磷酸(ATP)、2,3-二磷酸甘油酯(2,3-DPG)和丙二醛(MDA)检测试剂盒对上述指标进行检测.
结果: 保存中后期，EP-AS组和TAU-AS组红细胞溶血率均显著低于CPDA-1组(P<0.01)。EP-AS组MCV随保存时间延长而增加(r=0.71)，d 49时TAU-AS组MCV显著低于其余2组(P<0.05)。从d 14开始，EP-AS和TAU-AS组ATP和MDA浓度均显著高于CPDA-1组(P<0.01)；从d 7开始，EP-AS和TAU-AS组2,3-DPG浓度均显著高于CPDA-1组(P<0.01).
结论: 添加EP和TAU能显著降低4±2 ℃保存期间红细胞溶血率，且一定程度上抑制红细胞脂质过氧化水平和改善红细胞能量代谢。EP和TAU的作用机制可能与其抗氧化和膜保护作用有关，从而可以提高红细胞的保存质量并延长保存时间.
Keywords: ATP; erythrocyte; ethyl pyruvate; lipid peroxidation; taurine.