Objective: A dynamic gel loaded with lyophilized platelet-rich plasma-chitosan/difunctionalized polyethylene glycol (LPRP-CP) was prepared to investigate its hemostatic antibacterial and promoting wound healing of scald wounds through in vitro and in vivo experiments.
Methods: In this study, normal gauze/blank tablet (Ctrl), LPRP-CP, Chitosan HUCHUANG Powder(Chito P)and ChitoGauze XP PRO group (Chito G group) were set. The hemostatic effect and promoting healing effect of the four groups of materials were evaluated by establishing rabbit ear artery hemorrhage model and superficial Ⅱ° scalded model of skin on the back. The hemostatic time and bleeding amount were calculated and the gross and histological results of scald healing were observed. The antibacterial effect of the four groups of materials was evaluated by antibacterial test in vitro.
Results: In the rabbit ear arterial hemorrhage model, the hemostasis of all materials was successful. The hemostatic time of Ctrl, Chito P, LPRP-CP and Chito G groups was 213.33±38.30, 118.33±24.01, 115.00±8.37 and 111.67±11.69 s, respectively. The blood loss was 1233.83±992.27, 346.67±176.00, 193.33±121.47 and 147.50±80.66 mg, respectively. Compared with Ctrl, the hemostasis time of LPRP-CP, Chito P and Chito G group was significantly shorter (P<0.001), and the amount of blood loss of LPRP-CP and Chito G group was decreased (P<0.05). Compared with LPRP-CP, there were no significant differences in hemostatic time and blood loss between Chito P and Chito G group (P>0.05). In the model of superficial Ⅱ° scalded on the back of rabbit, the wound healing rate of LPRP-CP was faster than that of the other three groups at the same time, and the healing effect was perfect. In the antibacterial test in vitro, only LPRP-CP had better anti-S. aureus effect, and all groups had no anti-E. coli effect.
Conclusion: LPRP-CP is an excellent hemostatic material for superficial wounds, and has certain antibacterial and wound healing effects, which has a wide academic value and research prospects.
题目: 基于壳聚糖和聚乙二醇的新型负载冻干PRP止血抗菌凝胶.
目的: 制备负载冻干富血小板血浆-壳聚糖/双端苯甲醛基聚乙二醇动态凝胶（LPRP-CP）,通过体内外试验探讨其止血抗菌及促进烫伤创面愈合作用.
方法: 本研究设置普通纱布/空白药片对照（Ctrl）、壳聚糖护创粉（Chito P）、LPRP-CP和ChitoGauze XP PRO（Chito G）共4组。通过建立兔耳动脉出血模型及背部皮肤浅Ⅱ°烫伤模型，计算止血时间、出血量及观察烫伤愈合的大体及组织学结果，评价4组材料的止血效果和促愈合作用；通过体外抗菌试验，评价4组材料的抗菌效果.
结果: 在兔耳动脉出血模型中，各组材料均止血成功。Ctrl、Chito P、LPRP-CP和Chito G组止血时间分别为213.33±38.30、118.33±24.01、115.00±8.37和111.67±11.69 s，出血量分别为1233.83±992.27、346.67±176.00、193.33±121.47和147.50±80.66 mg。与Ctrl比较，Chito P、LPRP-CP和Chito G组的止血时间显著缩短（P＜0.001），LPRP-CP组和Chito G组的出血量减少（P＜0.05）；Chito P和Chito G组止血时间和出血量与LPRP-CP组比较均无统计学差异（P＞0.05）。在兔背部浅Ⅱ°烫伤模型中，LPRP-CP组的创面愈合速度均快于同一时间的其它3组，且愈合效果良好。在体外抗菌试验中，仅有LPRP-CP组有较好的抗金黄色葡萄球菌作用，所有组均无抗大肠杆菌的作用.
结论: LPRP-CP对浅表动脉损伤止血效果较好，同时兼具一定的抗菌和促进伤口愈合的作用，具有良好的应用前景.
Keywords: chitosan; hemostasis; platelet-rich plasma; polyethylene glycol.