Identification of 2,2',4,5,5'-Pentachlorobiphenyl (PCB101) metabolites and their transmission characteristics in silver crucian carp (Carassius auratus gibelio)

Shuai Wang; Meng-Yuan Wang; Yong-Fu Shi; Feng Han; Hong-Li Ye; You-Qiong Cai; Di Wu; Liang-Liang Tian; Xuan Zhang; Yun-Yu Tang

doi:10.1016/j.envpol.2022.119583

Identification of 2,2',4,5,5'-Pentachlorobiphenyl (PCB101) metabolites and their transmission characteristics in silver crucian carp (Carassius auratus gibelio)

Environ Pollut. 2022 Aug 15:307:119583. doi: 10.1016/j.envpol.2022.119583. Epub 2022 Jun 6.

Authors

Shuai Wang¹, Meng-Yuan Wang², Yong-Fu Shi³, Feng Han², Hong-Li Ye⁴, You-Qiong Cai⁴, Di Wu², Liang-Liang Tian⁴, Xuan Zhang⁴, Yun-Yu Tang⁴

Affiliations

¹ Fishery Products Quality Inspection and Test Centre (Shanghai), East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs of China, No. 300 Jungong Road, Yangpu District, Shanghai, 200090, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; Linquan County Agricultural Product Quality and Safety Inspection Station, Linquan, Anhui province, China.
² Fishery Products Quality Inspection and Test Centre (Shanghai), East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs of China, No. 300 Jungong Road, Yangpu District, Shanghai, 200090, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
³ Fishery Products Quality Inspection and Test Centre (Shanghai), East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs of China, No. 300 Jungong Road, Yangpu District, Shanghai, 200090, China. Electronic address: shiyf@ecsf.ac.cn.
⁴ Fishery Products Quality Inspection and Test Centre (Shanghai), East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs of China, No. 300 Jungong Road, Yangpu District, Shanghai, 200090, China.

PMID: 35680065
DOI: 10.1016/j.envpol.2022.119583

Abstract

Polychlorinated biphenyls (PCBs) have been attracting global concern due to their persistence and toxicity. However, the study on the metabolites of PCBs in freshwater fish is limited. In this study, the metabolites of 2,2',4,5,5'-Pentachlorobiphenyl (PCB101) in silver crucian carp (Carassius auratus gibelio) were identified for the first time. After intraperitoneal injection of PCB101 (2 mg/kg), the results showed that it could be metabolized to at least three types of metabolites, including hydroxylated (OH-), methoxylated (MeO-) and methyl sulfonated (MeSO₂-) PCB101. The OH- metabolites identified in most tissues were 3-OH-PCB101and 4-OH-PCB101, such as liver, gallbladder, blood and muscle. MeSO₂- metabolites identified in gallbladder, blood and brain were 3-MeSO₂-PCB101 and 4-MeSO₂-PCB101. Meanwhile, the MeO- metabolite identified in liver, gallbladder, blood and spleen of silver crucian carp was 4-MeO-PCB101. The investigation of the types and structures of PCB101 and its metabolites, as well as the tissue distribution and accumulation characteristics in silver crucian carp are beneficial to understand the transformation and metabolic mechanisms of PCBs in aquatic organisms. It is of great significance to identify potential pollution hazards of precursor compounds and their metabolites on aquatic products and ensure the quality and safety of aquatic products.

Keywords: GC-MS/MS; Identification; Metabolite; PCB101; Transfer characteristics.

MeSH terms

Animals
Carps* / metabolism
Goldfish
Polychlorinated Biphenyls* / metabolism

Substances

2,4,5,2',5'-pentachlorobiphenyl
Polychlorinated Biphenyls