Generation of an induced pluripotent stem cell line from a Chinese Han child with catecholaminergic polymorphic ventricular tachycardia

Wei Liu; Ting Liu; Tingting Xiao; Lijian Xie; Cuilan Hou; Xunwei Jiang

doi:10.1016/j.scr.2022.102811

Generation of an induced pluripotent stem cell line from a Chinese Han child with catecholaminergic polymorphic ventricular tachycardia

Stem Cell Res. 2022 Jul:62:102811. doi: 10.1016/j.scr.2022.102811. Epub 2022 May 11.

Authors

Wei Liu¹, Ting Liu², Tingting Xiao³, Lijian Xie¹, Cuilan Hou⁴, Xunwei Jiang⁵

Affiliations

¹ Department of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai 200062, China.
² Department of Pediatric Internal Medicine, Huashan Hospital, Fudan University, No.108 Luxiang Road, Shanghai 201900, China.
³ Department of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China.
⁴ Department of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China. Electronic address: houcl88@sina.cn.
⁵ Department of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355 Luding Road, Shanghai 200062, China. Electronic address: jiangxw@shchildren.com.cn.

PMID: 35679758
DOI: 10.1016/j.scr.2022.102811

Abstract

TECRL, first reported in a Sudanese family with catecholaminergic polymorphic ventricular tachycardia (CPVT) in 2016. TECRL, is an endoplasmic reticulum (ER) protein preferentially expressed in the heart, playing a role in cardiomyocyte calcium homeostasis. Using Sendaivirus-mediated reprogramming, we generated an induced pluripotent stem cell (iPSC) line from the CPVT patient's peripheral blood mononuclear cell. The iPSC exhibited stable amplification, expressed pluripotent markers, and differentiated spontaneously into three layers in vitro. Additionally, the iPSC line maintained a normal karyotype, retained the pathogenic TECRL mutation, and the cell resource facilitated a platform to explore the CPVT mechanisms related to TECRL mutations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Child
China
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear / pathology
Mutation
Tachycardia, Ventricular* / genetics
Tachycardia, Ventricular* / pathology

Supplementary concepts

Polymorphic catecholergic ventricular tachycardia