Umbilical Cord Mesenchymal Stem Cells Ameliorate Premature Ovarian Insufficiency in Rats

Meiliang Zhang; Tingting Xie; Weiyou Dai; Bo Zhao; Yaqin Zheng; Jianmiao Hu; Ruolang Pan; Liang Wang

doi:10.1155/2022/9228456

Umbilical Cord Mesenchymal Stem Cells Ameliorate Premature Ovarian Insufficiency in Rats

Evid Based Complement Alternat Med. 2022 May 30:2022:9228456. doi: 10.1155/2022/9228456. eCollection 2022.

Authors

Meiliang Zhang^{1

2}, Tingting Xie^{1

3}, Weiyou Dai⁴, Bo Zhao², Yaqin Zheng², Jianmiao Hu², Ruolang Pan⁴, Liang Wang¹

Affiliations

¹ Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
² Department of Obstetrics and Gynecology, Yiwu Maternity and Children Hospital, Yiwu Branch of Children's Hospital, Zhejiang University School of Medicine, Yiwu, Zhejiang, China.
³ Department of Obstetrics and Gynecology, The Women and Children Hospital of Dongyang, Dongyang Branch of Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Dongyang, Zhejiang, China.
⁴ Provincial Key Laboratory of Cell-Based Drug and Applied Technology Development, S-Evans Biosciences, Institute for Cell-Based Drug Development of Zhejiang Province, Hangzhou, Zhejiang, China.

Abstract

Premature ovarian insufficiency (POI) or premature ovarian failure (POF) is known as a state of hypergonadotropic hypogonadism. Stem cell therapy is expected to be used in the treatment of POI. The aim of the present study was to explore the feasibility and effectiveness of umbilical cord mesenchymal stem cell (UCMSC) transplantation for the treatment of POI in a rat model of POI induced by cyclophosphamide (CTX) injection. The ovarian function was examined by evaluating the weight of the ovary and body, estrus cycle, ovarian morphology, hormonal secretion, granulosa cell apoptosis, and fertility. The results showed that the ovarian function indicators of the modeled rats were comparable to those of the control rats after UCMSC transplantation, indicating that the ovarian function of the modeled rats recovered to a satisfactory extent. Our research may provide an experimental clue for the clinical application of UCMSC transplantation in the treatment of POI. Further experiments will focus on the detailed signaling pathway study of the molecular mechanisms of injury and repairment on the treatment with UCMSCs transplantation in the rat POI models.