Examining Neutrophil-Candida auris Interactions with Human Neutrophils Ex Vivo

Chad J Johnson; Jeniel E Nett

doi:10.1007/978-1-0716-2417-3_19

Examining Neutrophil-Candida auris Interactions with Human Neutrophils Ex Vivo

Methods Mol Biol. 2022:2517:243-250. doi: 10.1007/978-1-0716-2417-3_19.

Authors

Chad J Johnson¹, Jeniel E Nett^{2

3}

Affiliations

¹ Department of Medicine, University of Wisconsin, Madison, WI, USA.
² Department of Medicine, University of Wisconsin, Madison, WI, USA. jenett@medicine.wisc.edu.
³ Department of Medical Microbiology & Immunology, University of Wisconsin, Madison, WI, USA. jenett@medicine.wisc.edu.

Abstract

Neutrophils play a key role in controlling invasive fungal infections. These phagocytes engage and kill fungal pathogens through a variety of effector mechanisms. Here, we describe how to isolate human neutrophils for ex vivo study of neutrophil-Candida auris interactions. We detail assays to measure fungal killing, phagocytosis, and reactive oxygen species production.

Keywords: Candida auris; Ex vivo; Immunity; Killing; Neutrophil; Phagocyte; Phagocytosis; Reactive oxygen species.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Candida albicans*
Candida auris
Humans
Neutrophils* / microbiology
Phagocytosis
Reactive Oxygen Species

Substances

Reactive Oxygen Species

Abstract

Publication types

MeSH terms

Substances

Grants and funding