Measuring Gene Expression via mRNA Abundance in Candida auris

Abstract

The recently emerged human pathogenic yeast Candida auris has become a major global threat. As compared to other Candida species, C. auris often displays a high level of resistance to commonly used antifungals and poses additional therapeutic challenges. There is a great need to understand the molecular basis of its success as a drug-resistant human pathogen. The study of condition-specific gene expression can provide good cues of regulatory circuitry governing high drug resistance. Here, we describe the protocol of quantitative reverse transcription PCR (RT-qPCR) which can be conveniently employed as a highly reproducible method for measuring individual transcripts in C. auris cells.

Keywords: Candida auris; Drug resistance; Polymerase chain reaction; Quantitative reverse transcription; RNA; Transcriptome.