Cell viability assays are useful for assessing the efficacy of antifungal therapeutics and disinfection strategies in vitro. In recent years these assays have been fundamental for the testing of conventional and novel therapies against the nosocomial fungal pathogen Candida auris. Here we provide detailed descriptions of methods for assessing cellular viability of Candida auris in vitro, such as metabolic assays (XTT and resazurin), colony-forming unit counting, live/dead quantitative PCR, and fluorescent staining for microscopic analyses.
Keywords: Candida auris; Cell viability; Colony-forming units; Fluorescent staining; Live/dead qPCR; Resazurin; XTT.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.